Reference Report

Reference
Citation	Castillejo-Lopez, C., Haecker, U. (2005). The serine protease Sp7 is expressed in blood cells and regulates the melanization reaction in Drosophila. Biochem. Biophys. Res. Commun. 338(2): 1075--1082. (Export to RIS)
FlyBase ID	FBrf0189827
Publication Type	Research paper
PubMed ID	16256951
PubMed Abstract	Serine proteases play a central role in defense against pathogens by regulating processes such as blood clotting, melanization of injured surfaces, and proteolytic activation of signaling pathways involved in innate immunity. Here, we present the functional characterization of the Drosophila serine protease Sp7 (CG3006) by inducible RNA interference. We show that Sp7 is constitutively expressed in blood cells during embryonic and larval stages. Silencing of the gene impairs the melanization reaction upon injury. Our data demonstrate that Sp7 is required for phenoloxidase activation and its activity is restricted to a subclass of blood cells, the crystal cells. Transcriptional up-regulation of Sp7 was observed after clean, septic injury and in flies expressing an activated form of Toll; however, mutations in the Toll or the IMD pathway did not abolish expression of Sp7, indicating the existence of other regulatory pathways and/or independent basal transcription.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Biochem. Biophys. Res. Commun.
Title	Biochemical and Biophysical Research Communications
Publication Year	1959-
ISBN/ISSN	0006-291X
Data from Reference
Alleles (16)
	Avic\GFP^Scer\UAS.cUa Bc¹ dom^unspecified key¹ Rel^E20 Scer\GAL4^Act.PU Scer\GAL4^He.PZ Scer\GAL4^lz-gal4 Scer\GAL4^tub.PU Scer\GAL4^unspecified Sp7^{dsRNA.Scer\UAS} spz⁴ Tl⁸ tub¹ tub² w^+mC
Constructs (4)
	P{He-GAL4.Z} P{tub-GAL4.U} P{UAS-GFP.U} P{UAS-Sp7.dsRNA}
Genes (11)
	Avic\GFP Bc dom key Rel Scer\GAL4 Sp7 spz Tl tub w
Insertions (3)
	P{GawB}lz^gal4 P{UAS-Sp7.dsRNA}17a P{UAS-Sp7.dsRNA}42a
Natural transposons (1)
	P-element