Reference Report

Reference
Citation	Bainton, R.J., Tsai, L.T.Y., Schwabe, T., DeSalvo, M., Gaul, U., Heberlein, U. (2005). moody encodes two GPCRs that regulate cocaine behaviors and blood-brain barrier permeability in Drosophila. Cell 123(1): 145--156. (Export to RIS)
FlyBase ID	FBrf0189897
Publication Type	Research paper
PubMed ID	16213219
PubMed Abstract	We identified moody in a genetic screen for Drosophila mutants with altered cocaine sensitivity. Hypomorphic mutations in moody cause an increased sensitivity to cocaine and nicotine exposure. In contrast, sensitivity to the acute intoxicating effects of ethanol is reduced. The moody locus encodes two novel GPCRs, Moody-alpha and Moody-beta. While identical in their membrane-spanning domains, the two Moody proteins differ in their long carboxy-terminal domains, which are generated by use of alternative reading frames. Both Moody forms are required for normal cocaine sensitivity, suggesting that they carry out distinct but complementary functions. Moody-alpha and Moody-beta are coexpressed in surface glia that surround the nervous system, where they are actively required to maintain the integrity of the blood-brain barrier in the adult fly. We propose that a Moody-mediated signaling pathway functions in glia to regulate nervous system insulation and drug-related behaviors.
DOI
Related Publication(s)
Review	The blood-brain barrier - Lessons from moody flies. Daneman and Barres, 2005, Cell 123(1): 9--12 [FBrf0189905]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Aberrations (2)
	Df(1)moody-Δ17 Df(1)moody-Δ18
Alleles (11)
	moody^+t9.4 moody^+tgenα moody^+tgenβ moody^{dsRNA.Scer\UAS} moody^EP1529 moody^α.Scer\UAS moody^β.Scer\UAS Scer\GAL4^hs.PB Scer\GAL4^repo sdk^EP369 w^+mC
Constructs (7)
	P{GAL4-Hsp70.PB} P{moody^+t9.4} P{moody^+tgenα} P{moody^+tgenβ} P{UAS-moody.dsRNA} P{UAS-moody.α} P{UAS-moody.β}
Genes (10)
	Actn CG4313 CG4325 moody repo Scer\GAL4 sdk Sik2 Tre1 w
Insertions (9)
	P{EP}moody^EP1529 P{GAL4}repo P{moody^+t9.4}1 P{moody^+t9.4}2 P{moody^+t9.4}3 P{moody^+tgenα}1 P{moody^+tgenα}2 P{moody^+tgenβ}1 P{moody^+tgenβ}2
Natural transposons (1)
	P-element