Reference Report

Reference
Citation	Betschinger, J., Mechtler, K., Knoblich, J.A. (2006). Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells. Cell 124(6): 1241--1253. (Export to RIS)
FlyBase ID	FBrf0189901
Publication Type	Research paper
PubMed ID	16564014
PubMed Abstract	How stem cells generate both differentiating and self-renewing daughter cells is unclear. Here, we show that Drosophila larval neuroblasts-stem cell-like precursors of the adult brain-regulate proliferation by segregating the growth inhibitor Brat and the transcription factor Prospero into only one daughter cell. Like Prospero, Brat binds and cosegregates with the adaptor protein Miranda. In larval neuroblasts, both Brat and Prospero are required to inhibit self-renewal in one of the two daughter cells. While Prospero regulates cell-cycle gene transcription, Brat acts as a posttranscriptional inhibitor of dMyc. In brat or prospero mutants, both daughter cells grow and behave like neuroblasts leading to the formation of larval brain tumors. Similar defects are seen in lethal giant larvae (lgl) mutants where Brat and Prospero are not asymmetric. We have identified a molecular mechanism that may control self-renewal and prevent tumor formation in other stem cells as well.
DOI	10.1016/j.cell.2006.01.038
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (28)
	aPKC^ΔN.Scer\UAS brat^2L-150-11 brat^2L-192-9 brat^{hs.T:Zzzz\TAP} brat^{Scer\UAS.T:Avic\GFP-EGFP} CG17593^{hs.T:Zzzz\TAP} CG17593^{Scer\UAS.T:Avic\GFP-EGFP} dm^Scer\UAS.cZa insc^Mz1407 l(2)gl¹ l(2)gl⁴ mira^{126.hs.T:Zzzz\TAP} mira^{298.hs.T:Zzzz\TAP} mira^{300-830.hs.T:Zzzz\TAP} mira^{431.hs.T:Zzzz\TAP} mira^dsRNA.cBa mira^L44 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} Pi3K92E^{Scer\UAS.T:Hsap\MYC} pros¹⁷ Pten¹ Scer\GAL4^Act5C.PP Scer\GAL4^elav-C155 Scer\GAL4^insc-Mz1407 Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} T:Avic\GFP^EGFP w^+mC
Constructs (15)
	P{Dp110^D954A} P{GAL4-Act5C(-FRT).P} P{hs-brat.TAP} P{hs-CG17593.TAP} P{hs-mira.126.TAP} P{hs-mira.298.TAP} P{hs-mira.300-830.TAP} P{hs-mira.431.TAP} P{matα4-GAL-VP16} P{UAS-aPKC.ΔN} P{UAS-brat.GFP} P{UAS-CG17593.GFP} P{UAS-dm.Z} P{UAS-mCD8::GFP.L} P{WT-Dp110}
Genes (22)
	aPKC brat CG17593 CycE dm eve Fib foxo His3 insc l(2)gl mira Mmus\Cd8a Pi3K92E pros Pten Scer\GAL4 stau T:Zzzz\TAP w wor zfh1
Insertions (2)
	P{GawB}elav^C155 P{GawB}insc^Mz1407
Natural transposons (1)
	P-element