Reference Report

Reference
Citation	Chen, B.E., Kondo, M., Garnier, A., Watson, F.L., Puettmann-Holgado, R., Lamar, D.R., Schmucker, D. (2006). The molecular diversity of Dscam is functionally required for neuronal wiring specificity in Drosophila. Cell 125(3): 607--620. (Export to RIS)
FlyBase ID	FBrf0189904
Publication Type	Research paper
PubMed ID	16678102
PubMed Abstract	Alternative splicing of Dscam generates an enormous molecular diversity with maximally 38,016 different receptors. Whether this large diversity is required in vivo is currently unclear. We examined the role of Dscam in neuron-target recognition of single mechanosensory neurons, which connect with different target cells through multiple axonal branches. Analysis of Dscam null neurons demonstrated an essential role of Dscam for growth and directed extension of axon branches. Expression of randomly chosen single isoforms could not rescue connectivity but did restore basic axonal extension and rudimentary branching. Moreover, two Dscam alleles were generated that each reduced the maximally possible Dscam diversity to 22,176 isoforms. Reduction of Dscam diversity resulted in specific connectivity defects of mechanosensory neurons. Furthermore, the observed allele-specific phenotypes suggest functional differences among isoforms. Our findings provide evidence that a very large number of structurally unique receptor isoforms is required to ensure fidelity and precision of neuronal connectivity.
DOI	10.1016/j.cell.2006.03.034
Related Publication(s)
Review	Descrambling Dscam diversity. Bharadwaj and Kolodkin, 2006, Cell 125(3): 421--424 [FBrf0189902]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (14)
	Dscam^{1.30.30.2.A.Scer\UAS} Dscam^{1.34.30.2.Scer\UAS} Dscam^{7.6.19.2.Scer\UAS} Dscam²⁰ Dscam²¹ Dscam³³ Dscam⁰⁵⁵¹⁸ Dscam^R43 Dscam^R87 Dscam^ΔR265 Dscam^ΔR272 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^elav.PLu w^+mC
Constructs (6)
	P{5'PZ} P{GAL4-elav.L} P{UAS-Dscam.1.30.30.2} P{UAS-Dscam.1.34.30.2} P{UAS-Dscam.7.6.19.2} P{UAS-mCD8::GFP.L}
Genes (4)
	Dscam Mmus\Cd8a Scer\GAL4 w
Insertions (2)
	P{5'PZ}Dscam^ΔR272 P{PZ}Dscam⁰⁵⁵¹⁸
Natural transposons (1)
	P-element