Reference Report

Reference
Citation	Al-Anzi, B., Tracey, W.D., Benzer, S. (2006). Response of Drosophila to wasabi is mediated by painless, the fly homolog of mammalian TRPA1/ANKTM1. Curr. Biol. 16(10): 1034--1040. (Export to RIS)
FlyBase ID	FBrf0189995
Publication Type	Research paper
PubMed ID	16647259
PubMed Abstract	A number of repellent compounds produced by plants elicit a spicy or pungent sensation in mammals . In several cases, this has been found to occur through activation of ion channels in the transient receptor potential (TRP) family . We report that isothiocyanate (ITC), the pungent ingredient of wasabi, is a repellent to the insect Drosophila melanogaster, and that the painless gene, previously known to be required for larval nociception, is required for this avoidance behavior. A painless reporter gene is expressed in gustatory receptor neurons of the labial palpus, tarsus, and wing anterior margin, but not in olfactory receptor neurons, suggesting a gustatory role. Indeed, painless expression overlaps with a variety of gustatory-receptor gene reporters. Some, such as Gr66a, are known to be expressed in neurons that mediate gustatory repulsion . painless mutants are not taste blind; they show normal aversive gustatory behavior with salt and quinine and attractive responses to sugars and capsaicin. The painless gene is an evolutionary homolog of the mammalian "wasabi receptor" TRPA1/ANKTM1 , also thought to be involved in nociception. Our results suggest that the stinging sensation of isothiocyanate is caused by activation of an evolutionarily conserved molecular pathway that is also used for nociception.
DOI	10.1016/j.cub.2006.04.002
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (11)
	Avic\GFP^{Gr32a.3x.IRES.2x} Avic\GFP^{Gr47a.3x.IRES.2x} Avic\GFP^{Gr66a.3x.IRES.2x} Disc\RFP^Scer\UAS.cEa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} pain^+t8.5 pain^EP2451 pain^EP2621 pain^GAL4 pain^Scer\UAS.cTa Scer\GAL4^pain-GAL4
Constructs (6)
	P{Gr32a-GFP.IRES.GFP.IRES.GFP} P{Gr47a-GFP.IRES.GFP.IRES.GFP} P{Gr66a-GFP.IRES.GFP.IRES.GFP} P{pain-rescue} P{UAS-mCD8::GFP.L} P{UAS-pain.T}
Genes (5)
	Avic\GFP Disc\RFP Mmus\Cd8a pain Scer\GAL4
Insertions (4)
	P{EP}pain^EP2451 P{EP}pain^EP2621 P{GawB}pain^GAL4 P{UAS-pain.T}AR9
Natural transposons (1)
	P-element