Reference Report

Reference
Citation	Arbouzova, N.I., Bach, E.A., Zeidler, M.P. (2006). Ken & Barbie selectively regulates the expression of a subset of JAK/STAT pathway target genes. Curr. Biol. 16(1): 80--88. (Export to RIS)
FlyBase ID	FBrf0189996
Publication Type	Research paper
PubMed ID	16401426
PubMed Abstract	A limited number of evolutionarily conserved signal transduction pathways are repeatedly reused during development to regulate a wide range of processes. Here we describe a new negative regulator of JAK/STAT signaling and identify a potential mechanism by which the pleiotropy of responses resulting from pathway activation is generated in vivo. As part of a genetic interaction screen, we have identified Ken & Barbie (Ken) , which is an ortholog of the mammalian proto-oncogene BCL6 , as a negative regulator of the JAK/STAT pathway. Ken genetically interacts with the pathway in vivo and recognizes a DNA consensus sequence overlapping that of STAT92E in vitro. Tissue culture-based assays demonstrate the existence of Ken-sensitive and Ken-insensitive STAT92E binding sites, while ectopically expressed Ken is sufficient to downregulate a subset of JAK/STAT pathway target genes in vivo. Finally, we show that endogenous Ken specifically represses JAK/STAT-dependent expression of ventral veins lacking (vvl) in the posterior spiracles. Ken therefore represents a novel regulator of JAK/STAT signaling whose dynamic spatial and temporal expression is capable of selectively modulating the transcriptional repertoire elicited by activated STAT92E in vivo.
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Supplementary material	[FBrf0198579]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (2)
	Df(1)os1A2 Df(2R)Chi^g230
Alleles (18)
	aop^WT.GMR dome^G0367 dome^G0468 dome^{ΔCYT.Scer\UAS} ken¹ ken⁰²⁹⁷⁰ ken^k11035 ken^Scer\UAS.cAa ken^{Scer\UAS.T:Avic\GFP-EGFP} os^GMR.PB Ppyr\LUC^{2x2xphl.Stat92E.Ken} Ppyr\LUC^{2x2xphl.Stat92E.kenmut} Ppyr\LUC^{2x2xphl.Stat92Emut.kenmut} Ppyr\LUC^{phl.Stat92E.wt} Scer\GAL4^da.G32 Scer\GAL4^Sgs3.PD Stat92E⁰⁶³⁴⁶ Stat92E^HJ
Constructs (7)
	P{GAL4-da.G32} P{GMR-upd.B} P{GMR-yan^WT} P{Sgs3-GAL4.PD} P{UAS-dome.ΔCYT} P{UAS-ken.A} P{UAS-ken.GFP}
Genes (13)
	aop dome ken kni os Ppyr\LUC Scer\GAL4 Socs36E Stat92E Thiolase TM4SF trh vvl
Natural transposons (1)
	P-element