Reference Report

Reference
Citation	Horiuchi, D., Barkus, R.V., Pilling, A.D., Gassman, A., Saxton, W.M. (2005). APLIP1, a kinesin binding JIP-1/JNK scaffold protein, influences the axonal transport of both vesicles and mitochondria in Drosophila. Curr. Biol. 15(23): 2137--2141. (Export to RIS)
FlyBase ID	FBrf0190020
Publication Type	Research paper
PubMed ID	16332540
PubMed Abstract	In a genetic screen for Kinesin heavy chain (Khc)-interacting proteins, we identified APLIP1, a neuronally expressed Drosophila homolog of JIP-1, a JNK scaffolding protein . JIP-1 and its homologs have been proposed to act as physical linkers between kinesin-1, which is a plus-end-directed microtubule motor, and certain anterograde vesicles in the axons of cultured neurons . Mutation of Aplip1 caused larval paralysis, axonal swellings, and reduced levels of both anterograde and retrograde vesicle transport, similar to the effects of kinesin-1 inhibition. In contrast, Aplip1 mutation caused a decrease only in retrograde transport of mitochondria, suggesting inhibition of the minus-end microtubule motor cytoplasmic dynein . Consistent with dynein defects, combining heterozygous mutations in Aplip1 and Dynein heavy chain (Dhc64C) generated synthetic axonal transport phenotypes. Thus, APLIP1 may be an important part of motor-cargo linkage complexes for both kinesin-1 and dynein. However, it is also worth considering that APLIP1 and its associated JNK signaling proteins could serve as an important signaling module for regulating transport by the two opposing motors.
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Supplementary material	Supplemental data. [FBrf0191753]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (6)
	Df(3L)34ex5 Df(3L)Ar14-8 Df(3L)bab-PG Df(3L)Fpa1 Df(3L)Fpa2 Df(3L)ru-22
Alleles (15)
	Aplip1^+tHa Aplip1^EK4 Aplip1^{P483A.Scer\UAS} Aplip1^{P483L.Scer\UAS} Aplip1^{Scer\UAS.T:Avic\GFP-EGFP} Aplip1^{Scer\UAS.T:Zzzz\FLAG} Aplip1^{ΔKBD.Scer\UAS} Avic\GFP^{S65T.Scer\UAS.T:Ivir\HA1,T:Hsap\mito} Dhc64C^ek1 Khc²⁷ Klc^{Scer\UAS.T:Hsap\MYC} Klp64D^k5 n-syb^{Scer\UAS.cEa.T:Avic\GFP} Scer\GAL4^D42 T:Avic\GFP^EGFP
Constructs (7)
	P{Aplip1^+tHa} P{UAS-Aplip1.FLAG} P{UAS-Aplip1.ΔKBD} P{UAS-Klc.MYC} P{UAS-mito-HA-GFP.AP} P{UAS-n-syb-GFP.E} P{UASp-Aplip1.EGFP}
Genes (13)
	Aplip1 Avic\GFP Dhc64C Khc Klc Klp64D n-syb Scer\GAL4 Syt1 T:Avic\GFP T:Hsap\mito T:Hsap\MYC T:Zzzz\FLAG
Insertions (1)
	P{GawB}D42
Natural transposons (1)
	P-element