Reference Report

Reference
Citation	Koelzer, S., Klein, T. (2006). Regulation of expression of Vg and establishment of the dorsoventral compartment boundary in the wing imaginal disc by Suppressor of Hairless. Dev. Biol. 289(1): 77--90. (Export to RIS)
FlyBase ID	FBrf0190133
Publication Type	Research paper
PubMed ID	16307735
PubMed Abstract	The transcription factor Suppressor of Hairless (Su(H)) belongs to the CSL transcription factor family, which are the main transcriptional effectors of the Notch-signaling pathway. Su(H) is the only family member in the Drosophila genome and should therefore be the main transcriptional effector of the Notch pathway in this species. Despite this fact, in many developmental situations, the phenotype caused by loss of function of Su(H) is too weak for a factor that is supposed to mediate most or all aspects of Notch signaling. One example is the Su(H) mutant phenotype during the development of the wing, which is weaker in comparison to other genes required for Notch signaling. Another example is the complete absence of a phenotype upon loss of Su(H) function during the formation of the dorsoventral (D/V) compartment boundary, although the Notch pathway is required for this process. Recent work has shown that Su(H)/CBF1 has a second function as a transcriptional repressor, in the absence of the activity of the Notch pathway. As a repressor, Su(H) acts in a complex together with Hairless (H), which acts as a bridge to recruit the co-repressors Groucho and CtBP, and acts in a Notch-independent manner to prevent the transcription of target genes. This raises the possibility that a de-repression of target genes can occur in the case of loss if function of Su(H). Here, we show that the weak phenotype of Su(H) mutants during wing development and the absence of a phenotype during formation of the D/V compartment boundary are caused by the concomitant loss of the Notch-independent repressor function. This loss of the repressor function of Su(H) results in a de-repression of expression of target genes to a different degree in each process. Loss of Su(H) function during wing development results in a transient de-repression of expression of the selector gene vestigial (vg). We show that this residual expression of vg is responsible for the weaker mutant phenotype of Su(H) in the wing. During the formation of the D/V compartment boundary, de-repression of target genes seems to be sufficiently strong, to compensate the loss of Su(H) activity. Thus, de-repression of its target genes obscures the involvement of Su(H) in this process. Furthermore, we provide evidence that Dx does not signal in a Su(H)-independent manner as has been suggested previously.
DOI
Related Publication(s)
Review	Notch and affinity boundaries in Drosophila. Herranz and Milan, 2006, BioEssays 28(2): 113--116 [FBrf0189787]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(1)N-81k1
Alleles (24)
	ap^rK568 ap^UGO35 Avic\GFP^Scer\UAS.cYa dx^Scer\UAS.cMa Ecol\lacZ^vg.D:Venmut Ecol\lacZ^vg.int2.1 H^E31 H^Scer\UAS.cGa kuz^k01403 kuz^k01405 nct^A7 Psn^C1 Psn^I2 Scer\GAL4^dpp.blk1 Scer\GAL4^ptc-559.1 Scer\GAL4^sd-SG29.1 Scer\GAL4^αTub84B.PP Su(H)² Su(H)⁸ Su(H)^del47 Su(H)^Scer\UAS.cGa vg^83b27-R vg^null vg^Scer\UAS.cKa
Constructs (9)
	P{GAL4-dpp.blk1} P{GAL4-αTub84B(-FRT).P} P{UAS-DxA89} P{UAS-GFP.Y} P{UAS-H.G} P{UAS-Su(H).G} P{UAS-vg.K} P{vg(D/V)-lacZ} P{vg(D/Vmut)-lacZ}
Genes (16)
	ap Avic\GFP CtBP Dll dve dx Ecol\lacZ gro H kuz nct Psn Scer\GAL4 Su(H) vg wg
Insertions (5)
	P{GawB}ptc^559.1 P{GawB}sd^SG29.1 P{lacW}kuz^k01403 P{lacW}kuz^k01405 P{PZ}ap^rK568