Reference Report

Reference
Citation	Nairz, K., Rottig, C., Rintelen, F., Zdobnov, E., Moser, M., Hafen, E. (2006). Overgrowth caused by misexpression of a microRNA with dispensable wild-type function. Dev. Biol. 291(2): 314--324. (Export to RIS)
FlyBase ID	FBrf0190148
Publication Type	Research paper
PubMed ID	16443211
PubMed Abstract	MicroRNAs (miRNAs) represent an abundant class of non-coding RNAs that negatively regulate gene expression, primarily at the post-transcriptional level. miRNA genes are frequently located in proximity to fragile chromosomal sites associated with cancers and amplification of a miRNA cluster has been correlated with the etiology of lymphomas and solid tumors. The oncogenic potential of a miRNA polycistron has recently been demonstrated in vivo. Here, we show that misexpression of the Drosophila miRNA mirvana/mir-278 in the developing eye causes massive overgrowth, in part due to inhibition of apoptosis. A single base substitution affecting the mature miRNA blocks the gain-of-function phenotype but is not associated with a detectable reduction-of-function phenotype when homozygous. This result demonstrates that misexpressed miRNAs may acquire novel functions that cause unscheduled proliferation in vivo and thus exemplifies the potential of miRNAs to promote tumor formation.
DOI
Related Publication(s)
Personal communication to FlyBase	Details on mir-278[C610] and wts[5Y2]. Nairz, 2006.8.18, Details on mir-278[C610] and wts[5Y2]. [FBrf0199084]
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Secondary IDs
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(2R)Exel7137
Alleles (20)
	Avic\GFP^{EGFP.mir-278.αTub84B} Avic\GFP^{EGFP.αTub84B.B} EP2589^EP2589 grim^GMR.PC mir-278^EP1229 mir-278^EP2316 mir-278^EPC610 mir-278^{KN429.Scer\UAS} mir-278^{KN430.Scer\UAS} mir-278^{KN431.mirvana-1.Scer\UAS} mir-278^{KN447.Scer\UAS} mir-278^{KN448.mirvana-1.Scer\UAS} mir-278^mirvana-1 mir-278^mirvana-lj8 Scer\GAL4^GMR.PB Scer\GAL4^GMR.PF Scer\GAL4^hs.2sev Scer\GAL4^ptc-559.1 W^GMR.PG wts^5Y2
Constructs (14)
	P{EP.Scer\UAS.y⁺.H} P{EP} P{GAL4-Hsp70.sev} P{GAL4-ninaE.GMR} P{GMR-GAL4(FRT-)} P{GMR-grim} P{GMR-hid} P{Tub-EGFP.B} P{UAS-mir-278.KN429} P{UAS-mir-278.KN430</up>} P{UAS-mir-278.KN431.mirvana-1} P{UAS-mir-278.KN447} P{UAS-mir-278.KN448.mirvana-1} P{αTub84B-EGFP.mir-278}
Genes (11)
	Avic\GFP CG30465 Dpse\mir-278 EP2589 fus GMR grim mir-278 Scer\GAL4 W wts
Insertions (8)
	P{EP.Scer\UAS.y⁺.H}mir-278^EPC610 P{EP.Scer\UAS.y⁺.H}mir-278^mirvana-1 P{EP}EP2197 P{EP}EP2512 P{EP}EP2589^EP2589 P{EP}mir-278^EP1229 P{EP}mir-278^EP2316 P{GawB}ptc^559.1
Natural transposons (1)
	P-element