Reference Report

Reference
Citation	Bardin, A.J., Schweisguth, F. (2006). Bearded family members inhibit Neuralized-mediated endocytosis and signaling activity of Delta in Drosophila. Dev. Cell 10(2): 245--255. (Export to RIS)
FlyBase ID	FBrf0190191
Publication Type	Research paper
PubMed ID	16459303
PubMed Abstract	Endocytosis of Notch receptor ligands in signaling cells is essential for Notch receptor activation. In Drosophila, the E3 ubiquitin ligase Neuralized (Neur) promotes the endocytosis and signaling activity of the ligand Delta (Dl). In this study, we identify proteins of the Bearded (Brd) family as interactors of Neur. We show that Tom, a prototypic Brd family member, inhibits Neur-dependent Notch signaling. Overexpression of Tom inhibits the endocytosis of Dl and interferes with the interaction of Dl with Neur. Deletion of the Brd gene complex results in ectopic endocytosis of Dl in dorsal cells of stage 5 embryos. This defect in Dl trafficking is associated with ectopic expression of the single-minded gene, a direct Notch target gene that specifies the mesectoderm. We propose that inhibition of Neur by Brd proteins is important for precise spatial regulation of Dl signaling.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (4)
	Df(3L)Brd12 Df(3L)Brd15 Df(3L)Brd-C1 Df(3L)ED217
Alleles (13)
	E(spl)m2-BFM^Scer\UAS.cPa E(spl)m4-BFM^{Scer\UAS.T:Avic\GFP} E(spl)mα-BFM^Scer\UAS.cAa mib1^{C1205S.Scer\UAS} neur¹¹ neur^{C701S.Scer\UAS} Scer\GAL4^ap-md544 Scer\GAL4^dpp.PS Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^neur-P72 Scer\GAL4^ptc-559.1 Scer\GAL4^Ser.PGF Tom^Scer\UAS.cLa
Constructs (9)
	P{dpp-GAL4.PS} P{matα4-GAL-VP16} P{Ser-GAL4.GF} P{UAS-m2.P} P{UAS-m4.GFP} P{UAS-mib1.C1205S} P{UAS-mα.A} P{UAS-neur.C701S.B} P{UAS-Tom.L}
Genes (15)
	BobA BobB BobC Brd DCX-EMAP Dl E(spl)m2-BFM E(spl)m4-BFM E(spl)mα-BFM mib1 neur Ocho Scer\GAL4 sim Tom
Insertions (6)
	P{GAL4}neur^P72 P{GawB}ap^md544 P{GawB}ptc^559.1 P{matα4-GAL-VP16}67 P{XP}d02180 PBac{WH}DCX-EMAP^f02655
Natural transposons (1)
	P-element