Reference Report

Reference
Citation	Apitz, H., Struenkelnberg, M., de Couet, H.G., Fischbach, K.F. (2005). Single-minded, Dmef2, Pointed, and Su(H) act on identified regulatory sequences of the roughest gene in Drosophila melanogaster. Dev. Genes Evol. 215(9): 460--469. (Export to RIS)
FlyBase ID	FBrf0190252
Publication Type	Research paper
PubMed ID	16096801
PubMed Abstract	Roughest (Rst) is a cell adhesion molecule of the immunoglobulin superfamily that has multiple and diverse functions during the development of Drosophila melanogaster. The pleiotropic action of Rst is reflected by its complex and dynamic expression during the development of Drosophila. By an enhancer detection screen, we previously identified several cis-regulatory modules that mediate specific expression of the roughest gene in Drosophila developmental processes. To identify trans-regulators of rst expression, we used the Gal4/UAS system to screen for factors that were sufficient to activate Rst expression when ectopically expressed. By this method we identified the transcription factors Single-minded, Pointed.P1, and Su(H)-VP16. Furthermore, we showed that these factors and, in addition, Dmef2 are able to ectopically activate rst expression via the previously described rst cis-regulatory modules. This fact and the use of mutant analysis allocates the action of the transcription factors to specific developmental contexts. In the case of Sim, we could show that it regulates rst expression in the embryonic midline, but not in the optic lobes. Mutagenesis of Sim consensus binding sites in the regulatory module required for rst expression in the embryonic midline, abolished rst expression; indicating that the regulation of rst by Sim is direct.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Genes Evol.
Title	Development genes and evolution
Publication Year	1996-
ISBN/ISSN	0949-944X
Data from Reference
Alleles (28)
	abd-A^Scer\UAS.cGa aop^ACT.GMR ase^Scer\UAS.cBa ato^Scer\UAS.cJa B-H1^Scer\UAS.cSa B-H2^Scer\UAS.cSa Ecol\lacZ^rstF5 Ecol\lacZ^rstF6 Ecol\lacZ^Scer\UAS.cBa Egfr^{DN.Scer\UAS.cBa} Egfr^Scer\UAS.cBa ey^Scer\UAS.cHa Mef2^22-21 Mef2^Scer\UAS.cBa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} N^l1N-ts1 pnt^P1.Scer\UAS pnt^P2.Scer\UAS Scer\GAL4^GMR.PF Scer\GAL4^{rst.F6d-CME-I.II-mut} Scer\GAL4^sca-537.4 sim² sim^J1-47 sim^Scer\UAS.cXa Su(H)^{Scer\UAS.T:Hsap\MYC,T:Hsim\VP16} Su(H)^{Scer\UAS.T:Hsap\MYC} sv^Scer\UAS.cKa Ubx^Scer\UAS.cCa
Constructs (23)
	P{GAL4-ninaE.GMR} P{GMR-yan^ACT} P{rst.F5-lacZ} P{rst.F6-lacZ} P{rst-GAL4.F6d-CME-I/II-mut} P{UAS-abd-A.G} P{UAS-ase.B} P{UAS-ato.J} P{UAS-B-H1.S} P{UAS-B-H2.S} P{UAS-Egfr.B} P{UAS-Egfr.DN.B} P{UAS-ey.H} P{UAS-lacZ.B} P{UAS-mCD8::GFP.L} P{UAS-Mef2.B} P{UAS-pnt.P1} P{UAS-pnt.P2} P{UAS-sim.X} P{UAS-Su(H).myc} P{UAS-Su(H).VP16} P{UAS-sv.K} P{UAS-Ubx.Ia.C}
Genes (21)
	abd-A aop ase ato B-H1 B-H2 Dpse\GA17976 Ecol\lacZ Egfr ey Mef2 Mmus\Cd8a N pnt rst sca Scer\GAL4 sim Su(H) sv Ubx
Insertions (1)
	P{GawB}sca^537.4
Natural transposons (1)
	P-element