Reference Report

Reference
Citation	ten Bosch, J.R., Benavides, J.A., Cline, T.W. (2006). The TAGteam DNA motif controls the timing of Drosophila pre-blastoderm transcription. Development 133(10): 1967--1977. (Export to RIS)
FlyBase ID	FBrf0190276
Publication Type	Research paper
PubMed ID	16624855
PubMed Abstract	The Drosophila sex-determination switch gene Sex-lethal (Sxl) and the X-chromosome signal element genes (XSEs) that induce the female-specific expression of Sxl are transcribed extremely early in development when most of the genome of this organism is still silent. The DNA sequence CAGGTAG had been implicated in this pre-cellular blastoderm activation of sex-determination genes. A genome-wide computational search, reported here, suggested that CAGGTAG is not specific to early sex-determination genes, since it is over-represented upstream of most genes that are transcribed pre-cellular blastoderm, not just those involved in sex determination. The same search identified similarly over-represented, one-base-pair degenerate sequences as possible functional synonyms of CAGGTAG. We call these heptamers collectively, the TAGteam. Relevance of the TAGteam sequences to pre-cellular blastoderm transcription was established through analysis of TAGteam changes in Sxl, scute (an XSE), and the ;ventral repression element' of the pattern-formation gene zerknüllt. Decreasing the number of TAGteam sites retarded the onset of pre-blastoderm transcription, whereas increasing their number correlated with an advanced onset. Titration of repressors was thought to be the rate-limiting step determining the onset of such early transcription, but this TAGteam dose effect shows that activators must also play an important role in the timing of pre-blastoderm gene expression.
DOI	10.1242/dev.02373
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Secondary IDs	FBrf0195347
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (17)
	Ecol\lacZ^{Sxl.Pe.1.4.mutCAGGCAG} Ecol\lacZ^{Sxl.Pe.1.4.mutTAGteam} Ecol\lacZ^{Sxl.Pe.1.4.mutYAGGTAG} Ecol\lacZ^Sxl.Pe.1.4 Ecol\lacZ^Sxl.Pe Ecol\lacZ^{zen:eve.111bp.VRE} Ecol\lacZ^{zen:eve.250bp.VRE} Ecol\lacZ^{zen:eve.mut111bp.VRE} Ecol\lacZ^{zen:eve.mutVR:MSE} Ecol\lacZ^{zen:eve.VR:MSE} her¹ her^mat-1 sc^{1xXSE.CAGGcAG} sc^{1xXSE.CAGGTAG} sc^{3xXSE.CAGGTAG} sc^+t.XSE sc^M6
Constructs (13)
	@P{sc.1xXSE.CAGGcAG} P{lacZ<up>zen/eve.111bpVRE} P{lacZ<up>zen/eve.250bpVRE} P{lacZ^{zen/eve.600bpVRE}} P{lacZ<up>zen/eve.mut111bpVRE} P{lacZ^{zen/eve.mut600bpVRE}} P{sc.1xXSE.CAGGTAG} P{sc.3xXSE.CAGGTAG} P{sc.t.XSE} P{Sxl-Pe_1.4kb:lacZ} P{Sxl-Pe_1.4kb.mutCAGGCAG:lacZ} P{Sxl-Pe_1.4kb.mutTAGteam:lacZ} P{Sxl-Pe_1.4kb.mutYAGGTAG:lacZ}
Genes (42)
	bnk Dana\bnk Dana\sc Dana\sisA Dana\sna Dana\Sxl Dana\zen Dhyd\bnk Dmoj\bnk Dmoj\sc Dmoj\sisA Dmoj\sna Dmoj\Sxl Dmoj\zen Dpse\bnk Dpse\sc Dpse\sisA Dpse\sna Dpse\Sxl Dpse\zen Dvir\bnk Dvir\sc Dvir\sisA Dvir\sna Dvir\Sxl Dvir\zen Dwil\bnk Dyak\bnk Dyak\sc Dyak\sisA Dyak\sna Dyak\Sxl Dyak\zen Ecol\lacZ her Nrt sc sisA sna Sxl zen Ztub\bnk
Natural transposons (1)
	P-element