Reference Report

Reference
Citation	Coelho, C.M.A., Kolevski, B., Bunn, C., Walker, C., Dahanukar, A., Leevers, S.J. (2005). Growth and cell survival are unevenly impaired in pixie mutant wing discs. Development 132(24): 5411--5424. (Export to RIS)
FlyBase ID	FBrf0190282
Publication Type	Research paper
PubMed ID	16291791
PubMed Abstract	It is largely unknown how growth slows and then stops in vivo. Similar to most organs, Drosophila imaginal discs undergo a fast, near-exponential growth phase followed by a slow growth phase before final target size is reached. We have used a genetic approach to study the role of an ABC-E protein, Pixie, in wing disc growth. pixie mutants, like mutants in ribosomal proteins genes (known as Minutes), show severe developmental delay with relatively mild alterations in final body size. Intriguingly, pixie mutant wing imaginal discs show complex regional and temporal defects in growth and cell survival that are compensated to result in near-normal final size. In S2 cells, Pixie, like its yeast homolog RLI1, is required for translation. However, a comparison of the growth of eukaryotic translation initiation factor eIF4A and pixie mutant clones in wing discs suggests that only a subset of translation regulators, including pixie, mediate regional differences in growth and cell survival in wing discs. Interestingly, some of the regional effects on pixie mutant clone growth are enhanced in a Minute background. Our results suggest that the role of Pixie is not merely to allow growth, as might be expected for a translation regulator. Instead, Pixie also behaves as a target of putative constraining signals that slow disc growth during late larval life. We propose a model in which a balance of growth inhibitors and promoters determines tissue growth rates and cell survival. An alteration in this balance slows growth before final disc size is reached.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(3L)Scf-R11
Alleles (13)
	BacA\p35^Scer\UAS.cHa eIF-4a¹⁰⁰⁶ f^36a Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} pix^3c2 pix^3c3 pix^L17 pix^L24 pix^L35 RpL14¹ RpS3¹ Scer\GAL4^Bx-MS1096 Scer\GAL4^en-e16E
Constructs (2)
	P{Dp110^D954A} P{UAS-p35.H}
Genes (14)
	BacA\p35 CG1703 CG9281 CG9330 eIF-4a en f Pi3K92E pix RpL14 RpS3 Scer\GAL4 T:Hsap\MYC wg
Insertions (3)
	P{en2.4-GAL4}e16E P{GawB}Bx^MS1096 P{lacW}eIF-4a¹⁰⁰⁶