Reference Report

Reference
Citation	Han, Z., Yi, P., Li, X., Olson, E.N. (2006). Hand, an evolutionarily conserved bHLH transcription factor required for Drosophila cardiogenesis and hematopoiesis. Development 133(6): 1175--1182. (Export to RIS)
FlyBase ID	FBrf0190292
Publication Type	Research paper
PubMed ID	16467358
PubMed Abstract	The Hand gene family encodes highly conserved basic helix-loop-helix (bHLH) transcription factors that play crucial roles in cardiac and vascular development in vertebrates. In Drosophila, a single Hand gene is expressed in the three major cell types that comprise the circulatory system: cardioblasts, pericardial nephrocytes and lymph gland hematopoietic progenitors, but its function has not been determined. Here we show that Drosophila Hand functions as a potent transcriptional activator, and converting it into a repressor blocks heart and lymph gland formation. Disruption of Hand function by homologous recombination also results in profound cardiac defects that include hypoplastic myocardium and a deficiency of pericardial and lymph gland hematopoietic cells, accompanied by cardiac apoptosis. Targeted expression of Hand in the heart completely rescued the lethality of Hand mutants, and cardiac expression of a human HAND gene, or the caspase inhibitor P35, partially rescued the cardiac and lymph gland phenotypes. These findings demonstrate evolutionarily conserved functions of HAND transcription factors in Drosophila and mammalian cardiogenesis, and reveal a previously unrecognized requirement of Hand genes in hematopoiesis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(2L)Exel7046
Alleles (30)
	Avic\GFP^{HCH.Hand.hs.Pelican} BacA\p35^Scer\UAS.cHa Hand^{C.T:Scer\GAL4} Hand^{cHa.T:Hsim\VP16} Hand^cHa.T:Rep-en Hand^{cHa.T:Scer\GAL4} Hand^{cHa.ΔC.T:Hsim\VP16} Hand^{cHa.ΔN.T:Hsim\VP16} Hand^fl.cHa Hand^{ko.Scer\SceI.RS} Hand^ko Hand^{N.T:Scer\GAL4} Hand^{RRR.cHa.T:Hsim\VP16} Hand^RRR.cHa Hand^{RRR.T:Scer\GAL4} Hand^Scer\UAS.cHa Hand^{Scer\UAS.T:Hsim\VP16} Hand^{Scer\UAS.T:Rep-en} Hand^ΔC.cHa Hand^{ΔC.T:Scer\GAL4} Hand^ΔN.cHa Hand^{ΔN.T:Scer\GAL4} Hand^ΔNC.cHa Hand^{ΔNC.T:Scer\GAL4} Hsap\HAND2^Scer\UAS.cHa Ppyr\LUC^E6 Ppyr\LUC^{L8G4.Scer\UAS} Scer\GAL4^HCH.Hand Scer\GAL4^how-24B Scer\GAL4^twi.PG
Constructs (9)
	P{FRT(w^hs.Hand.Scer\SceI.RS</up>} P{GAL4-twi.G} P{GreenH-Pelican.HCH.Hand} P{HCH-GAL4} P{UAS-Hand.EnR} P{UAS-Hand.H} P{UAS-Hand.VP16} P{UAS-hHAND2.H} P{UAS-p35.H}
Genes (14)
	Avic\GFP BacA\p35 eve Hand Hsap\HAND2 Mef2 odd Ppyr\LUC prc Scer\GAL4 srp T:Hsim\VP16 T:Rep-en T:Scer\GAL4
Insertions (1)
	P{GawB}how^24B
Natural transposons (1)
	P-element