Reference Report

Reference
Citation	Tour, E., Hittinger, C.T., McGinnis, W. (2005). Evolutionarily conserved domains required for activation and repression functions of the Drosophila Hox protein Ultrabithorax. Development 132(23): 5271--5281. (Export to RIS)
FlyBase ID	FBrf0190326
Publication Type	Research paper
PubMed ID	16284118
PubMed Abstract	While testing the functions of deletion mutants in the Hox protein Ultrabithorax (Ubx), we found that the embryonic repression function of Ubx on Distal-less transcription in limb primordia is highly concentration dependent. The steep sigmoidal relationship between in vivo Ubx concentration and Distal-less repression is dependent on the Ubx YPWM motif. This suggests that Ubx cooperatively assembles a multi-protein repression complex on Distal-less regulatory DNA with the YPWM motif as a key protein-protein interface in this complex. Our deletion mutants also provide evidence for a transcriptional activation domain in the N-terminal 19 amino acids of Ubx. This proposed activation domain contains a variant of the SSYF motif that is found at the N termini of many Hox proteins, and is conserved in the activation domain of another Hox protein, Sex combs reduced. These results suggest that the N-terminal region containing the SSYF motif has been conserved in many Hox proteins for its role in transcriptional activation.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (12)
	Scer\GAL4^arm.PS Scr^Scer\UAS.cTa Scr^{ΔSSY.Scer\UAS} Ubx^{Ia.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ2-19.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ20-61.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ62-82.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ83-141.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ142-185.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ190-233.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ234-251.Scer\UAS.hs.T:Ivir\HA1} Ubx^{Ia.Δ252-280.Scer\UAS.hs.T:Ivir\HA1}
Constructs (12)
	P{GAL4-arm.S} P{UAS-Scer.ΔSSY} P{UAS-Scr.T} P{UAS-UbxIa.Δ2-19-HA} P{UAS-UbxIa.Δ20-61-HA} P{UAS-UbxIa.Δ62-82-HA} P{UAS-UbxIa.Δ83-141-HA} P{UAS-UbxIa.Δ142-185-HA} P{UAS-UbxIa.Δ190-233-HA} P{UAS-UbxIa.Δ234-251-HA} P{UAS-UbxIa.Δ252-280-HA} P{UAS-UbxIa-HA}
Genes (13)
	Antp CrebA Dfd Dll dpp fkh lab Scer\GAL4 Scr T:Ivir\HA1 tsh Ubx wg
Natural transposons (1)
	P-element