Reference Report

Reference
Citation	Page, A.R., Kovacs, A., Deak, P., Torok, T., Kiss, I., Dario, P., Bastos, C., Batista, P., Gomes, R., Ohkura, H., Russell, S., Glover, D.M. (2005). Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase. EMBO J. 24(24): 4304--4315. (Export to RIS)
FlyBase ID	FBrf0190363
Publication Type	Research paper
PubMed ID	16369566
PubMed Abstract	The highly condensed chromosomes and chromosome breaks in mitotic cells of a Drosophila mutant, spotted-dick/pita, are the consequence of defects in DNA replication. Reduction of levels of Spotted-dick protein, by either RNAi or mutation, leads to the accumulation of cells that have DNA content intermediate to 2N and 4N in proliferating tissues and also compromises endoreduplication in larval salivary glands. The Spotted-dick Zinc-finger protein is present in the nuclei of cells committed to proliferation but necessary in cells undertaking S phase. We show that Spotted-dick/Pita functions as a transcription factor and that, in cultured S2 cells, it is an activator of expression of some 30 genes that include the Orc4 gene, required for initiation of DNA replication. Chromatin immunoprecipitation indicates that it associates with the genes that it activates in S2 cells together with other sites that could represent genes activated in other tissues. We discuss the role of Spotted-dick in the coordination of cellular growth and DNA replication.
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Secondary IDs	FBrf0191763
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (1)
	Df(2R)bw5
Alleles (9)
	Dcp-1⁰²¹²¹ Dcp-1⁰⁸⁸⁵⁹ Dcp-1^k14408 Dcp-1^Scer\UAS.cPa pita⁰²¹²¹ pita⁰⁸⁸⁵⁹ pita^k14408 pita^Scer\UAS.cPb w^+mC
Constructs (2)
	P{UAS-Dcp-1.P} P{UAS-pita.P}
Genes (89)
	Act5C Amph APC7 Atox1 BubR1 c11.1 CG2091 CG2121 CG3038 CG3085 CG3117 CG3703 CG3918 CG4893 CG5149 CG5646 CG6234 CG6412 CG6693 CG7668 CG7824 CG7900 CG8500 CG9717 CG9723 CG10602 CG11586 CG11671 CG13707 CG14005 CG14545 CG14795 CG14968 CG15434 CG15715 CG16888 CG17681 CG18081 CG18528 CG30148 CG32536 CkIIα-i3 CLIP-190 ctp Dcp-1 Dd FKBP59 Gip gnu His3 HP5 inc Jabba Jra kat80 klar l(2)gl mRpL1 MTF-1 mthl8 MtnA mus209 nemy NimC4 nod Orc4 phl pita Prosap Pxn R Rca1 Ripα RpL17 RpS10a scaf skpA Snp Tcp-1ζ TfIIEα Timp Tmhs trbd Ubc-E2H Ufd1-like unk w αTub84B αTub84D
Insertions (3)
	P{lacW}Dcp-1^k14408 P{PZ}Dcp-1⁰²¹²¹ P{PZ}Dcp-1⁰⁸⁸⁵⁹
Natural transposons (1)
	P-element