Open Close
Reference
Citation
Müller, R., Hülsmeier, A.J., Altmann, F., Ten, H., Tiemeyer, M., Hennet, T. (2005). Characterization of mucin-type core-1 beta 1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster.  FEBS J. 272(17): 4295--4305.
FlyBase ID
FBrf0190470
Publication Type
Research paper
Abstract

Mucin type O-glycosylation is a widespread modification of eukaryotic proteins. The transfer of N-acetylgalactosamine to selected serine or threonine residues is catalyzed by a family of polypeptide N-acetylgalactosaminyltransferases localized in the Golgi apparatus. The most abundant elongation of O-glycans is the addition of a beta1-3 linked galactose by the core-1 beta1-3 galactosyltransferase (core-1 beta3GalT), thereby building the T-antigen or core-1 structure Gal(beta1-3)GalNAc(alpha1-O). We have isolated four Drosophila melanogaster cDNAs encoding proteins structurally similar to the human core-1 beta3GalT enzyme and expressed them as FLAG-tagged proteins in Sf9 insect cells. The identity of these D. melanogasterbeta3GalT enzymes with a core-1 beta3GalT activity was confirmed by utilization of MUC5AC mucin derived O-glycopeptide acceptors. In addition to the core-1 beta3GalT activity toward O-glycoprotein substrates, one member of this enzyme family showed a strong activity towards glycolipid acceptors, thereby building the core-1 terminated Nz6 glycosphingolipid. Transcripts of the embryonically expressed core-1 beta3GalTs were found in the maternally deposited mRNA, in salivary glands and in the amnioserosa. The presence of multiple core-1 beta3GalT genes in D. melanogaster suggests an increased complexity of core-1 O-glycan expression, which is possibly related to multiple developmental and physiological functions attributable to this class of glycans.

PubMed ID
PubMed Central ID
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0188602
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
FEBS J.
Title
FEBS Journal
Publication Year
2005-
ISBN/ISSN
1742-464X
Data From Reference
Gene Groups (1)
Genes (6)