Reference Report

Reference
Citation	Araki, M., Yu, H., Asano, M. (2005). A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo. Genes Dev. 19(20): 2458--2465. (Export to RIS)
FlyBase ID	FBrf0190535
Publication Type	Research paper
PubMed ID	16195415
PubMed Abstract	Regulated degradation plays a key role in setting the level of many factors that govern cell cycle progression. In Drosophila, the largest subunit of the origin recognition complex protein 1 (ORC1) is degraded at the end of M phase and throughout much of G1 by anaphase-promoting complexes (APC) activated by Fzr/Cdh1. We show here that none of the previously identified APC motifs targets ORC1 for degradation. Instead, a novel sequence, the O-box, is necessary and sufficient to direct Fzr/Cdh1-dependent polyubiquitylation in vitro and degradation in vivo. The O-box is similar to but distinct from the well characterized D-box. Finally, we show that O-box motifs in two other proteins, Drosophila Abnormal Spindle and Schizosaccharomyces pombe Cut2, contribute to Cdh1-dependent polyubiquitylation in vitro, suggesting that the O-box may mediate degradation of a variety of cell cycle factors.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (13)
	Avic\GFP^{Scer\UAS.T:SV40\nls2} Orc1^{1-248.Scer\UAS.T:Avic\GFP} Orc1^{242-556.3xmdb.Scer\UAS.T:Avic\GFP} Orc1^{242-556.Scer\UAS.T:Avic\GFP} Orc1^{C.Scer\UAS.T:Avic\GFP} Orc1^{mkb.3xmdb.Scer\UAS.T:Avic\GFP} Orc1^{mob.Scer\UAS.T:Avic\GFP} Orc1^{N.mkb.Scer\UAS.T:Avic\GFP} Orc1^{N.Scer\UAS.T:Avic\GFP} Orc1^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Act5C.PI Scer\GAL4^en-e16E Scer\GAL4^GMR.PF
Constructs (12)
	P{Act5C-GAL4} P{GAL4-ninaE.GMR} P{UAS-GFP.nls} P{UAS-Orc1.1-248.GFP} P{UAS-Orc1.242-556.3xmdb.GFP} P{UAS-Orc1.242-556.GFP} P{UAS-Orc1.C.GFP} P{UAS-Orc1.GFP} P{UAS-Orc1.mkb.3xmdb.GFP} P{UAS-Orc1.mob.GFP} P{UAS-Orc1.N.GFP} P{UAS-Orc1.N.mkb.GFP}
Genes (7)
	asp Avic\GFP fzy Orc1 rap Scer\GAL4 T:Avic\GFP
Insertions (1)
	P{en2.4-GAL4}e16E
Natural transposons (1)
	P-element