Reference Report

Reference
Citation	Geuking, P., Narasimamurthy, R., Basler, K. (2005). A genetic screen targeting the tumor necrosis factor/Eiger signaling pathway: identification of Drosophila TAB2 as a functionally conserved component. Genetics 171(4): 1683--1694. (Export to RIS)
FlyBase ID	FBrf0190722
Publication Type	Research paper
PubMed ID	16079232
PubMed Abstract	Signaling by tumor necrosis factors (TNFs) plays a prominent role in mammalian development and disease. To fully understand this complex signaling pathway it is important to identify all regulators and transduction components. A single TNF family member, Eiger, is encoded in the Drosophila genome, offering the possibility of applying genetic approaches for pursuing this goal. Here we present a screen for the isolation of novel genes involved in the TNF/Eiger pathway. On the basis of Eiger's ability to potently activate Jun-N-terminal kinase (JNK) and trigger apoptosis, we used the Drosophila eye to establish an assay for dominant suppressors of this activity. In a large-scale screen the Drosophila homolog of TAB2/3 (dTAB2) was identified as an essential component of the Eiger-JNK pathway. Genetic epistasis and biochemical protein-protein interaction assays assign an adaptor role to dTAB2, linking dTRAF1 to the JNKKK dTAK1, demonstrating a conserved mechanism of TNF signal transduction in mammals and Drosophila. Thus, in contrast to morphogenetic processes, such as dorsal closure of the embryo, in which the JNK pathway is activated by the JNKKK Slipper, Eiger uses the dTAB2-dTAK1 module to induce JNK signaling activity.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (2)
	Df(2R)Exel6069 Df(2R)P34
Alleles (74)
	bsk¹ bsk^G179 bsk^G258 bsk^G290 bsk^G368 bsk^G400 bsk^G418 bsk^G536 bsk^G577 bsk^G688 bsk^G760 egr^Scer\UAS.cMa G56^G56 G87^G87 hep^CA.Scer\UAS Ppyr\LUC^AP1 Scer\GAL4^GMR.PF Scer\GAL4^tub.PU slpr^unspecified Tab2^{C.Scer\UAS.T:Ivir\HA1} Tab2^G58 Tab2^G71 Tab2^G211 Tab2^G227 Tab2^G245 Tab2^G253 Tab2^G271 Tab2^G275 Tab2^G421 Tab2^G489 Tab2^G503 Tab2^G520 Tab2^G539 Tab2^G545 Tab2^G548 Tab2^G552 Tab2^G576 Tab2^G585 Tab2^G603 Tab2^G609 Tab2^G635 Tab2^G642 Tab2^G666 Tab2^G683 Tab2^G701 Tab2^G715 Tab2^G734 Tab2^G746 Tab2^G788 Tab2^G812 Tab2^G824 Tab2^G825 Tab2^G829 Tab2^G867 Tab2^G928 Tab2^G933 Tab2^G954 Tab2^G973 Tab2^G981 Tab2^{N.Scer\UAS.T:Ivir\HA1} Tab2^Scer\UAS.cGa Tab2^{Scer\UAS.T:Ivir\HA1} Tab2^{Scer\UAS.T:Zzzz\FLAG} Tab2^{Δcc.Scer\UAS.T:Ivir\HA1} Tab2^αTub84B.PG Tak1⁴ Tak1^+t15 Tak1^G14 Tak1^{Scer\UAS.T:Zzzz\FLAG} Tak1^unspecified Traf4^{Scer\UAS.cGa.T:Ivir\HA1} Traf6^{Scer\UAS.T:Ivir\HA1} w^+mC wgn^Scer\UAS.cMa
Constructs (6)
	P{dTAK1⁺} P{GAL4-ninaE.GMR} P{UAS-eiger.M} P{UAS-hep.CA} P{UAS-Tab2.G} P{αTub84B-Tab2.G}
Genes (23)
	bsk CG7461 CG11906 egr endoB FK506-bp2 G56 G87 hep msn Ppyr\LUC Rgk1 rib Scer\GAL4 slpr T:Ivir\HA1 T:Zzzz\FLAG Tab2 Tak1 Traf4 Traf6 w wgn
Natural transposons (1)
	P-element