Reference Report

Reference
Citation	Hammonds, A.S., Fristrom, J.W. (2006). Mutational analysis of Stubble-stubbloid gene structure and function in Drosophila leg and bristle morphogenesis. Genetics 172(3): 1577--1593. (Export to RIS)
FlyBase ID	FBrf0190727
Publication Type	Research paper
PubMed ID	16322506
PubMed Abstract	The Stubble-stubbloid (Sb-sbd) gene is required for ecdysone-regulated epithelial morphogenesis of imaginal tissues during Drosophila metamorphosis. Mutations in Sb-sbd are associated with defects in apical cell shape changes critical for the evagination of the leg imaginal disc and with defects in assembly and extension of parallel actin bundles in growing mechanosensory bristles. The Sb-sbd gene encodes a type II transmembrane serine protease (TTSP). Here we use a Sb-sbd transgenic construct to rescue both bristle and leg morphogenesis defects in Sb-sbd mutations. Molecular characterization of Sb-sbd mutations and rescue experiments with wild-type and modified Sb-sbd transgenic constructs show that the protease domain is required for both leg and bristle functions. Truncated proteins that express the noncatalytic domains without the protease have dominant effects in bristles but not in legs. Leg morphogenesis, but not bristle growth, is sensitive to Sb-sbd overexpression. Antibody localization of the Sb-sbd protein shows apical expression in elongating legs. Sb-sbd protein is found in the base and shaft in budding bristles and then concentrates at the growing tip when bristles are elongating rapidly. We propose a model whereby Sb-sbd helps coordinate proteolytic modification of extracellular matrix attachments with cytoskeletal changes in both legs and bristles.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (1)
	Df(3R)sbd105
Alleles (15)
	br^unspecified Sb¹ Sb^63b Sb⁷⁰ Sb^CD.hs Sb^CD-TM.hs Sb^{CD-TM-knot.hs} Sb^hs.PB Sb^S737A.hs Sb^sbd-2 Sb^sbd-201 Sb^Spi Sb^{Δprotease.hs} w^+mC zip^unspecified
Constructs (7)
	?{} P{hs-Sb.B} P{hs-Sb.CD} P{hs-Sb.CD-TM} P{hs-Sb.CD-TM-knot} P{hs-Sb.S737A} P{hs-Sb.Δprotease}
Genes (4)
	br Sb w zip
Insertions (4)
	?{}Sb¹ ?{}Sb^63b blood{}Sb^63b blood{}Sb⁷⁰
Natural transposons (2)
	blood P-element