Reference Report

Reference
Citation	Long, H., Cameron, S., Yu, L., Rao, Y. (2006). De novo GMP synthesis is required for axon guidance in Drosophila. Genetics 172(3): 1633--1642.
FlyBase ID	FBrf0190740
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	16322525
PubMed Abstract	Guanine nucleotides are key players in mediating growth-cone signaling during neural development. The supply of cellular guanine nucleotides in animals can be achieved via the de novo synthesis and salvage pathways. The de novo synthesis of guanine nucleotides is required for lymphocyte proliferation in animals. Whether the de novo synthesis pathway is essential for any other cellular processes, however, remains unknown. In a search for genes required for the establishment of neuronal connectivity in the fly visual system, we identify the burgundy (bur) gene as an essential player in photoreceptor axon guidance. The bur gene encodes the only GMP synthetase in Drosophila that catalyzes the final reaction of de novo GMP synthesis. Loss of bur causes severe defects in axonal fasciculation, retinotopy, and growth-cone morphology, but does not affect photoreceptor differentiation or retinal patterning. Similar defects were observed when the raspberry (ras) gene, encoding for inosine monophosphate dehydrogenase catalyzing the IMP-to-XMP conversion in GMP de novo synthesis, was mutated. Our study thus provides the first in vivo evidence to support an essential and specific role for de novo synthesis of guanine nucleotides in axon guidance.
Biosis	2006.368875
Zoological record
Associated Information
Comments
Text of personal communication
Associated files
Related Publications

Also Published As

Other Reference Information
Secondary IDs
Language of publication	English
Additional language(s) of abstract
ISBN
Place of publication
Published In
Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
Page range
Publisher
Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations (1)
	Df(2L)TW161
Alleles (17)
	Ecol\lacZ^{ro.T:Btau\MAPT} Mcm10^Scer\UAS.cLa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rac1^L89.Scer\UAS Rac1^N17.Scer\UAS Ret^Scer\UAS.cLa Scer\GAL4^GMR.PF Scer\GAL4^elav-C155 Scer\GAL80^αTub84B.PL bur¹ bur^Scer\UAS.cLa bur^e10 bur^k07130 ras¹¹ ras^G0002 ras^G0436 w^+mC
Constructs (9)
	P{GAL4-ninaE.GMR} P{UAS-Mcm10.L} P{UAS-Rac1.L89} P{UAS-Rac1.N17} P{UAS-Ret.L} P{UAS-bur.L} P{UAS-mCD8::GFP.L} P{ro.lacZ-tau.G} P{tubP-GAL80}
Genes (15)
	B Ecol\lacZ Mcm10 Mmus\Cd8a Rac1 Ret Scer\GAL4 Scer\GAL80 boss bur chp elav ras repo w
Insertions (2)
	P{GawB}elav^C155 P{lacW}bur^k07130
Natural transposons (1)
	P-element