Reference Report

Reference
Citation	Medina, P.M.B., Swick, L.L., Andersen, R., Blalock, Z., Brenman, J.E. (2006). A novel forward genetic screen for identifiyang mutations affecting larval neuronal dendrite development in Drosophila melanogaster. Genetics 172(4): 2325--2335. (Export to RIS)
FlyBase ID	FBrf0190747
Publication Type	Research paper
PubMed ID	16415365
PubMed Abstract	Vertebrate and invertebrate dendrites are information-processing compartments that can be found on both central and peripheral neurons. Elucidating the molecular underpinnings of information processing in the nervous system ultimately requires an understanding of the genetic pathways that regulate dendrite formation and maintenance. Despite the importance of dendrite development, few forward genetic approaches have been used to analyze the latest stages of dendrite development, including the formation of F-actin-rich dendritic filopodia or dendritic spines. We developed a forward genetic screen utilizing transgenic Drosophila second instar larvae expressing an actin, green fluorescent protein (GFP) fusion protein (actin::GFP) in subsets of sensory neurons. Utilizing this fluorescent transgenic reporter, we conducted a forward genetic screen of >4000 mutagenized chromosomes bearing lethal mutations that affected multiple aspects of larval dendrite development. We isolated 13 mutations on the X and second chromosomes composing 11 complementation groups affecting dendrite outgrowth/branching, dendritic filopodia formation, or actin::GFP localization within dendrites in vivo. In a fortuitous observation, we observed that the structure of dendritic arborization (da) neuron dendritic filopodia changes in response to a changing environment.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (2)
	Dp(1;Y)W39 Dp(1;Y)y²67g19.1
Alleles (15)
	Act5C^{Scer\UAS.T:Avic\GFP} aspn¹ bleb¹ bleb² bris¹ camo¹ capt^K304 lackluster¹ lent¹ mace¹ Moe^{GMA.Scer\UAS.T:Avic\GFP-S65T} polk¹ proj^unspecified Scer\GAL4^109(2)80 scnt¹
Constructs (2)
	P{UAS-Act5C.T:GFP} P{UAS-GMA}
Genes (15)
	Act5C aspn bleb bris camo capt futsch lackluster lent mace Moe polk proj Scer\GAL4 scnt
Insertions (1)
	P{GawB}109(2)80