Reference Report

Reference
Citation	Metaxakis, A., Oehler, S., Klinakis, A., Savakis, C. (2005). Minos as a genetic and genomic tool in Drosophila melanogaster. Genetics 171(2): 571--581.
FlyBase ID	FBrf0190750
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	15972463
PubMed Abstract	Much of the information about the function of D. melanogaster genes has come from P-element mutagenesis. The major drawback of the P element, however, is its strong bias for insertion into some genes (hotspots) and against insertion into others (coldspots). Within genes, 5'-UTRs are preferential targets. For the successful completion of the Drosophila Genome Disruption Project, the use of transposon vectors other than P will be necessary. We examined here the suitability of the Minos element from Drosophila hydei as a tool for Drosophila genomics. Previous work has shown that Minos, a member of the Tc1/mariner family of transposable elements, is active in diverse organisms and cultured cells; it produces stable integrants in the germ line of several insect species, in the mouse, and in human cells. We generated and analyzed 96 Minos integrations into the Drosophila genome and devised an efficient "jump-starting" scheme for production of single insertions. The ratio of insertions into genes vs. intergenic DNA is consistent with a random distribution. Within genes, there is a statistically significant preference for insertion into introns rather than into exons. About 30% of all insertions were in introns and approximately 55% of insertions were into or next to genes that have so far not been hit by the P element. The insertion sites exhibit, in contrast to other transposons, little sequence requirement beyond the TA dinucleotide insertion target. We further demonstrate that induced remobilization of Minos insertions can delete nearby sequences. Our results suggest that Minos is a useful tool complementing the P element for insertional mutagenesis and genomic analysis in Drosophila.
Biosis	2006.104676
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Language of publication	English
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Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
Page range
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Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations
	In(2LR)O
Alleles
	Avic\GFP^E.3xP3 Dhyd\Minos\T^hs.PF Scer\GAL4^ET1 w^+mC
Balancers
	CyO CyO-MiT2.4
Genes
	AR-2 Ace Antp Avic\GFP Btk29A CG11147 CG12367 CG12508 CG13408 CG13474 CG13624 CG17019 CG17562 CG17648 CG18599 CG1988 CG30497 CG31120 CG31150 CG31410 CG31646 CG31998 CG32457 CG32549 CG4467 CG5156 CG5191 CG5346 CG5613 CG6520 CG6718 CG7091 CG7294 CG7628 DIP2 Dhyd\Minos\T Dys Gos28 Oatp74D Or82a Pka-C3 Proct RluA-1 Scer\GAL4 Tsp66A Ucp4B cher cln3 cpo cpx dac daw dlp dnc ex heph kuz robo3 tRNA:CR31382 w
Other
	pHSS6hsMi2 pMi{ET1}
Natural transposons
	Dhyd\Minos
Insertions
	P{hsILMiT}2.4
Other
	Mi{ET1} Mi{PR1} P{CaSpeR-4} P{hsILMiT}