Reference Report

Reference
Citation	Metaxakis, A., Oehler, S., Klinakis, A., Savakis, C. (2005). Minos as a genetic and genomic tool in Drosophila melanogaster. Genetics 171(2): 571--581. (Export to RIS)
FlyBase ID	FBrf0190750
Publication Type	Research paper
PubMed ID	15972463
PubMed Abstract	Much of the information about the function of D. melanogaster genes has come from P-element mutagenesis. The major drawback of the P element, however, is its strong bias for insertion into some genes (hotspots) and against insertion into others (coldspots). Within genes, 5'-UTRs are preferential targets. For the successful completion of the Drosophila Genome Disruption Project, the use of transposon vectors other than P will be necessary. We examined here the suitability of the Minos element from Drosophila hydei as a tool for Drosophila genomics. Previous work has shown that Minos, a member of the Tc1/mariner family of transposable elements, is active in diverse organisms and cultured cells; it produces stable integrants in the germ line of several insect species, in the mouse, and in human cells. We generated and analyzed 96 Minos integrations into the Drosophila genome and devised an efficient "jump-starting" scheme for production of single insertions. The ratio of insertions into genes vs. intergenic DNA is consistent with a random distribution. Within genes, there is a statistically significant preference for insertion into introns rather than into exons. About 30% of all insertions were in introns and approximately 55% of insertions were into or next to genes that have so far not been hit by the P element. The insertion sites exhibit, in contrast to other transposons, little sequence requirement beyond the TA dinucleotide insertion target. We further demonstrate that induced remobilization of Minos insertions can delete nearby sequences. Our results suggest that Minos is a useful tool complementing the P element for insertional mutagenesis and genomic analysis in Drosophila.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (1)
	In(2LR)O
Alleles (4)
	Avic\GFP^E.3xP3 Dhyd\Minos\T^hs.PF Scer\GAL4^ET1 w^+mC
Balancers (1)
	CyO-MiT2.4
Constructs (16)
	3xP3-EGFP.SV40 001363 001371 001372 Mi_3'(298) Mi_5'(350) Mi{ET1} Mi{PR1} NotI P{CaSpeR-4} P{hsILMiT} pHSS6hsMi2 pHSS6-MCS pMi{ET1} pMi{PR1} pSL-3xP3-EGFP
Genes (59)
	Ace Antp AR-2 Avic\GFP Btk29A CG1988 CG4467 CG5191 CG5346 CG5613 CG6520 CG7091 CG7294 CG11147 CG13408 CG13474 CG13624 CG17019 CG17562 CG17648 CG18599 CG30497 CG31646 CG31998 CG32457 CG32549 CG42329 CG42575 CG43861 cher cln3 cpo cpx cv-d dac daw Dhyd\Minos\T DIP2 dlp dnc Dys ex Gos28 Hen1 heph iPLA2-VIA kuz Npc2e Oatp74D Or82a Pka-C3 Proct RluA-1 robo3 Scer\GAL4 tRNA:CR31382 Tsp66A Ucp4B w
Insertions (1)
	P{hsILMiT}2.4
Libraries/Collections (1)
	TI_set_Mi{ET1}.MB
Natural transposons (1)
	Dhyd\Minos