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Citation
Chen, Z., Robin, C., Damiano, J., Lydall, J., Lumb, C., Smith, K., Blasetti, A., Daborn, P.J., Heckel, D., McKenzie, J.A., Batterham, P. (2006). Positional cloning of a cyromazine resistance gene in Drosophila melanogaster.  Insect Mol. Biol. 15(2): 181--186.
FlyBase ID
FBrf0190854
Publication Type
Research paper
Abstract

Cyromazine is an effective insecticide used to control dipteran insects. Its precise mode of action is yet to be determined, although it has been suggested that it interferes with the hormone system, sclerotization of the cuticle, or nucleic acid metabolism. To understand the way in which cyromazine acts, we have positionally cloned a cyromazine resistance gene from Drosophila melanogaster. Six cyromazine resistance alleles had previously been generated by ethyl methanasulphonate treatment. Two of these failed to complement each other and here we identify them as having independent non-sense mutations in CG32743, which is an ortholog of Smg1 of worms and mammals and encodes a phosphatidylinositol kinase-like kinase (PIKK). RNAi experiments confirm that cyromazine resistance can be achieved by knocking down CG32743. These are the first cyromazine resistant mutations identified at the nucleotide level. In mammals Smg1 phosphorylates P53 in response to DNA damage. This finding supports the hypothesis that cyromazine interferes with nucleic acid metabolism.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Insect Mol. Biol.
    Title
    Insect Molecular Biology
    Publication Year
    1992-
    ISBN/ISSN
    0307-6975 0962-1075 1365-2583
    Data From Reference
    Alleles (5)
    Genes (6)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (2)