Reference Report

Reference
Citation	Sano, H., Renault, A.D., Lehmann, R. (2005). Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2. J. Cell Biol. 171(4): 675--683. (Export to RIS)
FlyBase ID	FBrf0190943
Publication Type	Research paper
PubMed ID	16301333
PubMed Abstract	In most organisms, primordial germ cells (PGCs) arise far from the region where somatic gonadal precursors (SGPs) are specified. Although PGCs in general originate as a single cluster of cells, the somatic parts of the gonad form on each site of the embryo. Thus, to reach the gonad, PGCs not only migrate from their site of origin but also split into two groups. Taking advantage of high-resolution real-time imaging, we show that in Drosophila melanogaster PGCs are polarized and migrate directionally toward the SGPs, avoiding the midline. Unexpectedly, neither PGC attractants synthesized in the SGPs nor known midline repellents for axon guidance were required to sort PGCs bilaterally. Repellent activity provided by wunen (wun) and wunen-2 (wun-2) expressed in the central nervous system, however, is essential in this migration process and controls PGC survival. Our results suggest that expression of wun/wun-2 repellents along the migratory paths provides faithful control over the sorting of PGCs into two gonads and eliminates PGCs left in the middle of the embryo.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (4)
	Df(1)NP5 Df(2R)NYX-D15 Df(2R)wun-GL Df(3L)H99
Alleles (24)
	abd-A^46.51 BacA\p35^Scer\UAS.cHa Ecol\lacZ^1A121 Ecol\lacZ^Scer\UAS.cUa Hmgcr^26.31 Hmgcr^{Scer\UAS.cvDa} lea^robo2-1 lea^robo2-4 lea^robo2-8 Moe^{DB.nos.nos3'UTR,T:Avic\GFP-EGFP} NetA^{Scer\UAS.T:Hsap\MYC} NetB^{Scer\UAS.T:Hsap\MYC} nos^bcd.3UTR robo¹ Scer\GAL4^9-201av Scer\GAL4^69B Scer\GAL4^how-24B Scer\GAL4^nos.PG sim² sli² sli^Scer\UAS.cKa W^Scer\UAS.cSa wun2^{Scer\UAS.T:Hsap\MYC} wun^CE
Constructs (9)
	P{nos-Moe.EGFP.nos3'UTR} P{UAS-hid.S} P{UAS-Hmgcr.vD} P{UAS-lacZ.U} P{UAS-NetrinA} P{UAS-NetrinB} P{UAS-p35.H} P{UAS-sli.K} P{UAS-wun2.Myc}
Genes (19)
	abd-A BacA\p35 Ecol\lacZ grim Hmgcr lea Moe NetA NetB nos robo rpr Scer\GAL4 sim sli vas W wun wun2
Insertions (3)
	P{GawB}69B P{GawB}how^24B P{lArB}1A121
Natural transposons (1)
	P-element