Reference Report

Reference
Citation	Zavortink, M., Contreras, N., Addy, T., Bejsovec, A., Saint, R. (2005). Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster. J. Cell Sci. 118(22): 5381--5392. (Export to RIS)
FlyBase ID	FBrf0190967
Publication Type	Research paper
PubMed ID	16280552
PubMed Abstract	A central question in understanding cytokinesis is how the cleavage plane is positioned. Although the positioning signal is likely to be transmitted via the anaphase microtubule array to the cell cortex, exactly how the microtubule array determines the site of contractile ring formation remains unresolved. By analysing tum/RacGAP50C mutant Drosophila embryos we show that cells lacking Tum do not form furrows and fail to localise the key cytokinetic components Pebble (a RhoGEF), Aurora B kinase, Diaphanous, Pav-KLP and Anillin. The GAP activity of Tum is required for cytokinesis: in its absence cytokinesis fails early even though Tum is present on microtubules at the cell equator where the furrow should form. Disruption of the Pebble-interacting domain leaves Tum localised to the cell equator on cortically associated microtubules, again with no evidence of furrowing. These data support a model in which Tum/RacGAP, via its interaction with Pbl, provides a critical link between the anaphase microtubule spindle and cytokinetic furrow formation in Drosophila cells.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (1)
	Df(2R)Exel7128
Alleles (15)
	pbl^{Scer\UAS.T:Avic\GFP} pbl^unspecified Scer\GAL4^en-e16E Scer\GAL4^prd.RG1 Scer\GAL4^unspecified tum¹ tum³⁴⁷ tum^AR2 tum^DH15 tum^{dsRNA.Scer\UAS.cSa} tum^{Scer\UAS.Z.T:Hsap\MYC} tum^{ΔEIE.Scer\UAS} tum^{ΔPav.Scer\UAS} tum^{ΔPbl.Scer\UAS} tum^{ΔYRL.Scer\UAS}
Constructs (8)
	P{GAL4-prd.F} P{UAS-pbl::GFP} P{UAS-RacGAP50C.RNAi} P{UAS-tum.MYC.Z} P{UAS-tum.ΔEIE} P{UAS-tum.ΔPav} P{UAS-tum.ΔPbl} P{UAS-tum.ΔYRL}
Genes (8)
	dia ial pav pbl Scer\GAL4 scra T:Hsap\MYC tum
Insertions (1)
	P{en2.4-GAL4}e16E
Natural transposons (1)
	P-element