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Citation
Alonso, J., Rodriguez, J.M., Baena-Lopez, L.A., Santaren, J.F. (2005). Characterization of the Drosophila melanogaster mitochondrial proteome.  J. Proteome Res. 4(5): 1636--1645.
FlyBase ID
FBrf0191062
Publication Type
Research paper
Abstract
We have combined high-resolution two-dimensional (2-D) gel electrophoresis with mass spectrometry with the aim of identifying proteins represented in the 2-D gel database of Drosophila melanogaster mitochondria. First, we purified mitochondria from third instar Drosophila larvae and constructed a high-resolution 2-D gel database containing 231 silver-stained polypeptides. Next, we carried out preparative 2-D PAGE to isolate some of the polypeptides and characterize them by MALDI-TOF analysis. Using this strategy, we identified 66 mitochondrial spots in the database, and in each case confirmed their identity by MALDI-TOF/TOF analysis. In addition, we generated antibodies against two of the mitochondrial proteins as tools for characterizing the organelle.
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Proteome Res.
    Title
    Journal of Proteome Research
    Publication Year
    2002
    ISBN/ISSN
    1535-3893
    Data From Reference