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| Citation | Alonso, J., Rodriguez, J.M., Baena-Lopez, L.A., Santaren, J.F. (2005). Characterization of the Drosophila melanogaster mitochondrial proteome. J. Proteome Res. 4(5): 1636--1645. (Export to RIS) | ||
| FlyBase ID | FBrf0191062 | ||
| Publication Type | Research paper | ||
| PubMed ID | 16212416 | ||
| PubMed Abstract | We have combined high-resolution two-dimensional (2-D) gel electrophoresis with mass spectrometry with the aim of identifying proteins represented in the 2-D gel database of Drosophila melanogaster mitochondria. First, we purified mitochondria from third instar Drosophila larvae and constructed a high-resolution 2-D gel database containing 231 silver-stained polypeptides. Next, we carried out preparative 2-D PAGE to isolate some of the polypeptides and characterize them by MALDI-TOF analysis. Using this strategy, we identified 66 mitochondrial spots in the database, and in each case confirmed their identity by MALDI-TOF/TOF analysis. In addition, we generated antibodies against two of the mitochondrial proteins as tools for characterizing the organelle. | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | J. Proteome Res. | ||
| Title | Journal of Proteome Research | ||
| Publication Year | 2002 | ||
| ISBN/ISSN | 1535-3893 | ||
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Data from Reference
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| Genes (46)
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