Reference Report

Reference
Citation	Capelson, M., Corces, V.G. (2005). The ubiquitin ligase dTopors directs the nuclear organization of a chromatin insulator. Mol. Cell 20(1): 105--116. (Export to RIS)
FlyBase ID	FBrf0191177
Publication Type	Research paper
PubMed ID	16209949
PubMed Abstract	Chromatin insulators are gene regulatory elements implicated in the establishment of independent chromatin domains. The gypsy insulator of D. melanogaster confers its activity through a protein complex that consists of three known components, Su(Hw), Mod(mdg4)2.2, and CP190. We have identified a factor, Drosophila Topoisomerase I-interacting RS protein (dTopors) that interacts with the insulator protein complex and is required for gypsy insulator function. In the absence of Mod(mdg4)2.2, nuclear clustering of insulator complexes is disrupted and insulator activity is compromised. Overexpression of dTopors in the mod(mdg4)2.2 null mutant rescues insulator activity and restores the formation of nuclear insulator bodies. dTopors associates with the nuclear lamina, and mutations in lamin disrupt dTopors localization as well as nuclear organization and activity of the gypsy insulator. Thus, dTopors appears to be involved in the establishment of chromatin organization through its ability to mediate the association of insulator complexes with a fixed nuclear substrate.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell
Title	Molecular Cell
Publication Year	1997-
ISBN/ISSN	1097-2765 1097-4164
Data from Reference
Aberrations (1)
	Df(2R)P34
Alleles (13)
	bi^D11 ct⁶ Lam⁰⁴⁶⁴³ mod(mdg4)^ul Scer\GAL4^Act.PU Scer\GAL4^hs.PB su(Hw)^{5.3.T:Avic\GFP} T:Avic\GFP^EGFP Topors^{C118S.Scer\UAS.T:Avic\GFP-EGFP} Topors^{dsRNA.Scer\UAS} Topors^{Scer\UAS.T:Avic\GFP-EGFP} w^+mC y²
Constructs (5)
	P{GAL4-Hsp70.PB} P{su(Hw)^{5.3.T:Avic\GFP}} P{UAS-EGFP-Topors.C118S} P{UAS-EGFP-Topors} P{UAS-Topors.RNAi}
Genes (11)
	bi Cp190 ct gypsy\su(Hw)BR Lam mod(mdg4) Scer\GAL4 su(Hw) Topors w y
Insertions (1)
	gypsy{}y²