Reference Report

Reference
Citation	Hozumi, S., Maeda, R., Taniguchi, K., Kanai, M., Shirakabe, S., Sasamura, T., Speder, P., Noselli, S., Aigaki, T., Murakami, R., Matsuno, K. (2006). An unconventional myosin in Drosophila reverses the default handedness in visceral organs. Nature 440(7085): 798--802. (Export to RIS)
FlyBase ID	FBrf0191242
Publication Type	Research paper
PubMed ID	16598258
PubMed Abstract	The internal organs of animals often have left-right asymmetry. Although the formation of the anterior-posterior and dorsal-ventral axes in Drosophila is well understood, left-right asymmetry has not been extensively studied. Here we find that the handedness of the embryonic gut and the adult gut and testes is reversed (not randomized) in viable and fertile homozygous Myo31DF mutants. Myo31DF encodes an unconventional myosin, Drosophila MyoIA (also referred to as MyoID in mammals; refs 3, 4), and is the first actin-based motor protein to be implicated in left-right patterning. We find that Myo31DF is required in the hindgut epithelium for normal embryonic handedness. Disruption of actin filaments in the hindgut epithelium randomizes the handedness of the embryonic gut, suggesting that Myo31DF function requires the actin cytoskeleton. Consistent with this, we find that Myo31DF colocalizes with the cytoskeleton. Overexpression of Myo61F, another myosin I (ref. 4), reverses the handedness of the embryonic gut, and its knockdown also causes a left-right patterning defect. These two unconventional myosin I proteins may have antagonistic functions in left-right patterning. We suggest that the actin cytoskeleton and myosin I proteins may be crucial for generating left-right asymmetry in invertebrates.
DOI	10.1038/nature04625
Related Publication(s)
Review	Left-right asymmetry: actin-myosin through the looking glass. Baum, 2006, Curr. Biol. 16(13): R502--R504 [FBrf0193117]
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
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Parent Publication
Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Aberrations (1)
	Df(2L)J2
Alleles (20)
	Cdc42^F89.Scer\UAS Moe^{Scer\UAS.T:Avic\GFP} Myo31DF^{dsRNA.Sym.Scer\UAS} Myo31DF^L152 Myo31DF^Scer\UAS.cSa Myo31DF^{Scer\UAS.T:Avic\GFP} Myo31DF^souther Myo61F^{dsRNA.Scer\UAS} Myo61F^GS9889 Myo61F^Scer\UAS.cHa Rac1^N17.Scer\UAS Rho1^N19.Scer\UAS Scer\GAL4^48Y Scer\GAL4^Act5C.PI Scer\GAL4^byn-Gal4 Scer\GAL4^da.G32 Scer\GAL4^nos.PG Scer\GAL4^NP2432 Scer\GAL4^NP5021 Scer\GAL4^{Scer\FRT.Act5C}
Constructs (13)
	P{AyGAL4} P{GAL4-da.G32} P{GAL4-nos.NGT} P{GSV6} P{Sym-UAS-Myo31DF} P{UAS-Cdc42.F89} P{UAS-Moe::GFP} P{UAS-Myo31DF.GFP} P{UAS-Myo31DF.S} P{UAS-Myo61F.dsRNA} P{UAS-Myo61F.H} P{UAS-Rac1.N17} P{UAS-Rho1.N19}
Genes (7)
	Cdc42 Moe Myo31DF Myo61F Rac1 Rho1 Scer\GAL4
Insertions (7)
	gypsy{}Myo31DF^souther P{GawB}48Y P{GawB}byn^Gal4 P{GawB}NP2432 P{GawB}NP5021 P{GSV6}GS14508 P{GSV6}Myo61F^GS9889
Natural transposons (2)
	gypsy P-element