Reference Report

Reference
Citation	Speder, P., Adam, G., Noselli, S. (2006). Type ID unconventional myosin controls left-right asymmetry in Drosophila. Nature 440(7085): 803--807. (Export to RIS)
FlyBase ID	FBrf0191256
Publication Type	Research paper
PubMed ID	16598259
PubMed Abstract	Breaking left-right symmetry in Bilateria embryos is a major event in body plan organization that leads to polarized adult morphology, directional organ looping, and heart and brain function. However, the molecular nature of the determinant(s) responsible for the invariant orientation of the left-right axis (situs choice) remains largely unknown. Mutations producing a complete reversal of left-right asymmetry (situs inversus) are instrumental for identifying mechanisms controlling handedness, yet only one such mutation has been found in mice (inversin) and snails. Here we identify the conserved type ID unconventional myosin 31DF gene (Myo31DF) as a unique situs inversus locus in Drosophila. Myo31DF mutations reverse the dextral looping of genitalia, a prominent left-right marker in adult flies. Genetic mosaic analysis pinpoints the A8 segment of the genital disc as a left-right organizer and reveals an anterior-posterior compartmentalization of Myo31DF function that directs dextral development and represses a sinistral default state. As expected of a determinant, Myo31DF has a trigger-like function and is expressed symmetrically in the organizer, and its symmetrical overexpression does not impair left-right asymmetry. Thus Myo31DF is a dextral gene with actin-based motor activity controlling situs choice. Like mouse inversin, Myo31DF interacts and colocalizes with beta-catenin, suggesting that situs inversus genes can direct left-right development through the adherens junction.
DOI	10.1038/nature04623
Related Publication(s)
Review	Left-right asymmetry: actin-myosin through the looking glass. Baum, 2006, Curr. Biol. 16(13): R502--R504 [FBrf0193117]
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
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Parent Publication
Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Aberrations (2)
	Df(2L)Exel7048 Df(2L)J3
Alleles (20)
	Disc\RFP^{Scer\UAS.T:Myr-Src64B} Myo31DF^{dsRNA.Sym.Scer\UAS} Myo31DF^K1 Myo31DF^K2 Myo31DF^KG02246 Myo31DF^Scer\UAS.cSa Myo31DF^{Scer\UAS.T:Avic\GFP} Myo31DF^{ΔIQ.Scer\UAS} Rfx^unspecified Scer\GAL4^Abd-B-LDN Scer\GAL4^cad-md509 Scer\GAL4^dpp.blk1 Scer\GAL4^elav.PLu Scer\GAL4^en-e16E Scer\GAL4^hh-Gal4 Scer\GAL4^{Myo31DF-NP1548} Scer\GAL4^ptc-559.1 Scer\GAL4^tsh-md621 Scer\GAL80^ts.αTub84B w^+mC
Constructs (9)
	P{GAL4-dpp.blk1} P{GAL4-elav.L} P{SUPor-P} P{Sym-UAS-Myo31DF} P{tubP-GAL80^ts} P{UAS-Myo31DF.GFP} P{UAS-Myo31DF.S} P{UAS-Myo31DF.ΔIQ} P{UAS-myr-mRFP}
Genes (15)
	arm CG6094 CG6144 CG7384 CG7456 CG13144 Disc\RFP Fatp Grip75 Myo31DF Rfx RluA-2 Scer\GAL4 Scer\GAL80 w
Insertions (8)
	P{en2.4-GAL4}e16E P{GAL4}hh^Gal4 P{GawB}Abd-B^LDN P{GawB}cad^md509 P{GawB}Myo31DF^NP1548 P{GawB}ptc^559.1 P{GawB}tsh^md621 P{SUPor-P}Myo31DF^KG02246
Natural transposons (1)
	P-element