Reference Report

Reference
Citation	Hiramoto, M., Hiromi, Y. (2006). ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin. Nat. Neurosci. 9(1): 58--66. (Export to RIS)
FlyBase ID	FBrf0191279
Publication Type	Research paper
PubMed ID	16341212
PubMed Abstract	Networks in the CNS consist of neural modules that are connected in a repetitive array. Whereas individual modules contain guidance information along which axons track within the unit, these guidance cues hinder axon extension across module boundaries. We investigated how axons solve this 'boundary problem' by analyzing the longitudinal connections of neuromeres in Drosophila melanogaster. The initial trajectory of the longitudinal axons is guided by Netrin, which is localized on commissural axons by its receptor, Frazzled. The Netrin cue on the commissure of the next segment can act as a barrier to longitudinal axons, inhibiting their extension and misguiding them contralaterally along the commissure. We show that, before reaching the segmental boundary, the longitudinal axons' responsiveness to Netrin presented on the commissure is suppressed by Roundabout (ROBO), through counteracting Gq signaling. The absence of suppression causes the robo phenotype: longitudinal axons project toward the midline, as if running around a roundabout (rotary).
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Neurosci.
Title	Nature Neuroscience
Publication Year	1998-
ISBN/ISSN	1097-6256
Data from Reference
Aberrations (1)
	Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9
Alleles (15)
	comm^Scer\UAS.cUa Ecol\lacZ^fkh-F223 Ecol\lacZ^{Scer\UAS.T:Btau\MAPT} fra³ fra^{ΔC.Scer\UAS.T:Hsap\MYC} Gαq^{Q203L.Scer\UAS} Mmus\Itpr1^{mut.sponge.Scer\UAS.T:Avic\GFP} Mmus\Itpr1^{sponge.Scer\UAS.T:Avic\GFP} robo¹ robo^{Scer\UAS.T:Hsap\MYC} Scer\GAL4^15J2 Scer\GAL4⁶⁰⁵ Scer\GAL4^insc-Mz1407 sli^GA20 sli^Scer\UAS.cKa
Constructs (8)
	P{UAS-comm.U} P{UAS-fra.ΔC} P{UAS-GFP-IP3-sponge.mut} P{UAS-GFP-IP3-sponge} P{UAS-Gα49B.Q203L} P{UAS-robo.Myc} P{UAS-sli.K} P{UAS-tau-lacZ.B}
Genes (13)
	comm Ecol\lacZ Fas2 fra futsch Gαq Mmus\Itpr1 NetA NetB robo Scer\GAL4 sli T:Avic\GFP
Insertions (4)
	P{GAL4}605 P{GawB}15J2 P{GawB}insc^Mz1407 P{lacZ}fkh^F223
Natural transposons (1)
	P-element