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Wagh, D.A., Rasse, T.M., Asan, E., Hofbauer, A., Schwenkert, I., Duerrbeck, H., Buchner, S., Dabauvalle, M.C., Schmidt, M., Qin, G., Wichmann, C., Kittel, R., Sigrist, S.J., Buchner, E. (2006). Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila.  Neuron 49(6): 833--844.
FlyBase ID
FBrf0191329
Publication Type
Research paper
Abstract

Neurotransmitters are released at presynaptic active zones (AZs). In the fly Drosophila, monoclonal antibody (MAB) nc82 specifically labels AZs. We employ nc82 to identify Bruchpilot protein (BRP) as a previously unknown AZ component. BRP shows homology to human AZ protein ELKS/CAST/ERC, which binds RIM1 in a complex with Bassoon and Munc13-1. The C terminus of BRP displays structural similarities to multifunctional cytoskeletal proteins. During development, transcription of the bruchpilot locus (brp) coincides with neuronal differentiation. Panneural reduction of BRP expression by RNAi constructs permits a first functional characterization of this large AZ protein: larvae show reduced evoked but normal spontaneous transmission at neuromuscular junctions. In adults, we observe loss of T bars at active zones, absence of synaptic components in electroretinogram, locomotor inactivity, and unstable flight (hence "bruchpilot"-crash pilot). We propose that BRP is critical for intact AZ structure and normal-evoked neurotransmitter release at chemical synapses of Drosophila.

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Erratum

Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila (vol 49, pg 833, 2006).
Wagh et al., 2006, Neuron 51(2): 275 [FBrf0191909]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference
    Alleles (9)
    Genes (18)
    Natural transposons (1)
    Insertions (6)
    Experimental Tools (3)
    Transgenic Constructs (6)