Reference Report

Reference
Citation	Yang, Z., Edenberg, H.J., Davis, R.L. (2005). Isolation of mRNA from specific tissues of Drosophila by mRNA tagging. Nucleic Acids Res. 33(17): e148. (Export to RIS)
FlyBase ID	FBrf0191351
Publication Type	Research paper
PubMed ID	16204451
PubMed Abstract	To study the function of specific cells or tissues using genomic tools like microarray analyses, it is highly desirable to obtain mRNA from a homogeneous source. However, this is particularly challenging for small organisms, like Caenorhabditis elegans and Drosophila melanogaster. We have optimized and applied a new technique, mRNA tagging, to isolate mRNA from specific tissues of D.melanogaster. A FLAG-tagged poly(A)-binding protein (PABP) is expressed in a specific tissue and mRNA from that tissue is thus tagged by the recombinant PABP and separated from mRNA in other tissues by co-immunoprecipitation with a FLAG-tag specific antibody. The fractionated mRNA is then amplified and used as probe in microarray experiments. As a test system, we employed the procedures to identify genes expressed in Drosophila photoreceptor cells. We found that most known photoreceptor cell-specific mRNAs were identified by mRNA tagging. Furthermore, at least 11 novel genes have been identified as enriched in photoreceptor cells. mRNA tagging is a powerful general method for profiling gene expression in specific tissues and for identifying tissue-specific genes.
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Secondary IDs	FBrf0191578
Language of Publication	English
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Publication Type	Journal
Abbreviation	Nucleic Acids Res.
Title	Nucleic Acids Research
Publication Year	1974-
ISBN/ISSN	0305-1048
Data from Reference
Alleles (9)
	eya^unspecified Hsap\PABPC1^{Scer\UAS.T:Zzzz\FLAG} pAbp^{Scer\UAS.T:Zzzz\FLAG} Scer\GAL4^Act5C.PI Scer\GAL4^elav-C155 Scer\GAL4^ey-OK107 Scer\GAL4^GMR.PF Scer\GAL4^Tab2-201Y Scer\GAL4^unspecified
Constructs (4)
	P{Act5C-GAL4} P{GAL4-ninaE.GMR} P{UAS-dPF} P{UAS-hPF}
Genes (55)
	Arr1 Arr2 boss CdsA CG6656 CG7220 CG10638 CG18598 chp Cpn Cpr72Ec cry Cry dlg1 eya Fbxl4 gl Gαq Gβ76C Gγ30A hdc Hsap\PABPC1 inaC inaD inaF-A inaF-B inaF-C inaF-D lqf msta ninaA ninaC ninaE norpA pAbp Pdh rdgA rdgC Rh2 Rh3 Rh4 Rh5 Rh6 rtp Scer\GAL4 Sh so st t T:Zzzz\FLAG Tequila trp trpl Tsp42Ej Xport
Insertions (11)
	P{Act5C-GAL4}25FO1 P{GAL4-ninaE.GMR}12 P{GawB}elav^C155 P{GawB}ey^OK107 P{GawB}Tab2^201Y P{UAS-hPABP-FLAG}B P{UAS-hPABP-FLAG}D P{UAS-hPABP-FLAG}Y P{UAS-PABP-FLAG}A3 P{UAS-PABP-FLAG}B2 P{UAS-PABP-FLAG}D
Natural transposons (1)
	P-element