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Citation
Yang, Z., Edenberg, H.J., Davis, R.L. (2005). Isolation of mRNA from specific tissues of Drosophila by mRNA tagging.  Nucleic Acids Res. 33(17): e148.
FlyBase ID
FBrf0191351
Publication Type
Research paper
Abstract

To study the function of specific cells or tissues using genomic tools like microarray analyses, it is highly desirable to obtain mRNA from a homogeneous source. However, this is particularly challenging for small organisms, like Caenorhabditis elegans and Drosophila melanogaster. We have optimized and applied a new technique, mRNA tagging, to isolate mRNA from specific tissues of D.melanogaster. A FLAG-tagged poly(A)-binding protein (PABP) is expressed in a specific tissue and mRNA from that tissue is thus tagged by the recombinant PABP and separated from mRNA in other tissues by co-immunoprecipitation with a FLAG-tag specific antibody. The fractionated mRNA is then amplified and used as probe in microarray experiments. As a test system, we employed the procedures to identify genes expressed in Drosophila photoreceptor cells. We found that most known photoreceptor cell-specific mRNAs were identified by mRNA tagging. Furthermore, at least 11 novel genes have been identified as enriched in photoreceptor cells. mRNA tagging is a powerful general method for profiling gene expression in specific tissues and for identifying tissue-specific genes.

PubMed ID
PubMed Central ID
PMC1243647 (PMC) (EuropePMC)
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Secondary IDs
  • FBrf0191578
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Nucleic Acids Res.
Title
Nucleic Acids Research
Publication Year
1974-
ISBN/ISSN
0305-1048
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