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Citation
Staudt, N., Molitor, A., Somogyi, K., Mata, J., Curado, S., Eulenberg, K., Meise, M., Siegmund, T., Haeder, T., Hilfiker, A., Broenner, G., Ephrussi, A., Rorth, P., Cohen, S.M., Fellert, S., Chung, H.R., Piepenburg, O., Schäfer, U., Jäckle, H., Vorbrüggen, G. (2005). Gain-of-function screen for genes that affect Drosophila muscle pattern formation.  PLoS Genet. 1(4): e55.
FlyBase ID
FBrf0191391
Publication Type
Research paper
Abstract
This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
PubMed ID
PubMed Central ID
PMC1270011 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Genet.
    Title
    PLoS Genetics
    Publication Year
    2005-
    ISBN/ISSN
    1553-7404 1553-7390
    Data From Reference
    Alleles (11)
    Genes (42)
    Datasets (2)
    Natural transposons (1)
    Insertions (502)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Experimental Tools (1)
    Transgenic Constructs (7)