Reference Report

Reference
Citation	Aboobaker, A.A., Tomancak, P., Patel, N., Rubin, G.M., Lai, E.C. (2005). Drosophila microRNAs exhibit diverse spatial expression patterns during embryonic development. Proc. Natl. Acad. Sci. U.S.A. 102(50): 18017--18022. (Export to RIS)
FlyBase ID	FBrf0191424
Publication Type	Research paper
PubMed ID	16330759
PubMed Abstract	MicroRNAs (miRNAs) are an extensive class of regulatory RNA whose specific functions in animals are generally unknown. Although computational methods have identified many potential targets of miRNAs, elucidating the spatial expression patterns of miRNAs is necessary to identify the sites of miRNA action. Here, we report the spatial patterns of miRNA transcription during Drosophila embryonic development, as revealed by in situ hybridization to nascent miRNA transcripts. We detect expression of 15 "stand-alone" miRNA loci and 9 intronic miRNA loci, which collectively represent 38 miRNA genes. We observe great variety in the spatial patterns of miRNA transcription, including preblastoderm stripes, in aspects of the central and peripheral nervous systems, and in cellular subsets of the mesoderm and endoderm. We also describe an intronic miRNA (miR-7) whose expression pattern is distinct from that of its host mRNA (bancal), which demonstrates that intronic miRNAs can be subject to independent cis-regulatory control. Intriguingly, the expression patterns of several fly miRNAs are analogous to those of their vertebrate counterparts, suggesting that these miRNAs may have ancient roles in animal patterning.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (3)
	bl^EP954 mir-7^Scer\UAS.cLa Scer\GAL4^ptc-559.1
Constructs (1)
	P{UAS-mir-7.L}
Genes (40)
	abd-A bl CG10479 CG32085 mir-1 mir-2a-1 mir-2a-2 mir-2b-1 mir-2c mir-3 mir-4 mir-5 mir-6-1 mir-6-2 mir-6-3 mir-7 mir-8 mir-10 mir-11 mir-12 mir-13b-1 mir-13b-2 mir-14 mir-31a mir-92a mir-124 mir-184 mir-263b mir-274 mir-279 mir-281-1 mir-281-2 mir-283 mir-286 mir-304 mir-307a mir-309 mir-315 mir-316 Scer\GAL4
Insertions (1)
	P{GawB}ptc^559.1