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Citation
Gerber, A.P., Luschnig, S., Krasnow, M.A., Brown, P.O., Herschlag, D. (2006). Genome-wide identification of mRNAs associated with the translational regulator Pumilio in Drosophila melanogaster.  Proc. Natl. Acad. Sci. U.S.A. 103(12): 4487--4492.
FlyBase ID
FBrf0191437
Publication Type
Research paper
Abstract
Genome-wide identification of RNAs associated with RNA-binding proteins is crucial for deciphering posttranscriptional regulatory systems. PUMILIO is a member of the evolutionary conserved Puf-family of RNA-binding proteins that repress gene expression posttranscriptionally. We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we purified PUMILIO from whole adult flies and embryos and analyzed associated mRNAs by using DNA microarrays. Distinct sets comprising hundreds of mRNAs were associated with PUMILIO at the two developmental stages. Many of these mRNAs encode functionally related proteins, supporting a model for coordinated regulation of posttranscriptional modules by specific RNA-binding proteins. We identified a characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO, and the sufficiency of this motif for interaction with PUMILIO was confirmed by RNA pull-down experiments with biotinylated synthetic RNAs. The RNA motif strikingly resembles the one previously identified for Puf3p, one of five Saccharomyces cerevisiae Puf proteins; however, proteins encoded by the associated mRNAs in yeast and Drosophila do not appear to be related. The results suggest extensive posttranscriptional regulation by PUMILIO and uncover evolutionary features of this conserved family of RNA-binding proteins.
PubMed ID
PubMed Central ID
PMC1400586 (PMC) (EuropePMC)
Related Publication(s)
Note
mRNA regulation by Puf domain proteins.
Wharton and Aggarwal, 2006, Sci. STKE 2006(354): pe27 [FBrf0193010]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (3)
    Genes (39)
    Physical Interactions (5)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (1)