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Reference Report
| Reference | |||
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| Citation | Lebedeva, L.A., Nabirochkina, E.N., Kurshakova, M.M., Robert, F., Krasnov, A.N., Evgen'ev, M.B., Kadonaga, J.T., Georgieva, S.G., Tora, L. (2005). Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation. Proc. Natl. Acad. Sci. U.S.A. 102(50): 18087--18092. (Export to RIS) | ||
| FlyBase ID | FBrf0191449 | ||
| Publication Type | Research paper | ||
| PubMed ID | 16330756 | ||
| PubMed Abstract | The presence of general transcription factors and other coactivators at the Drosophila hsp70 gene promoter in vivo has been examined by polytene chromosome immunofluorescence and chromatin immunoprecipitation at endogenous heat-shock loci or at a hsp70 promoter-containing transgene. These studies indicate that the hsp70 promoter is already occupied by TATA-binding protein (TBP) and several TBP-associated factors (TAFs), TFIIB, TFIIF (RAP30), TFIIH (XPB), TBP-free/TAF-containg complex (GCN5 and TRRAP), and the Mediator complex subunit 13 before heat shock. After heat shock, there is a significant recruitment of the heat-shock transcription factor, RNA polymerase II, XPD, GCN5, TRRAP, or Mediator complex 13 to the hsp70 promoter. Surprisingly, upon heat shock, there is a marked diminution in the occupancy of TBP, six different TAFs, TFIIB, and TFIIF, whereas there is no change in the occupancy of these factors at ecdysone-induced loci under the same conditions. Hence, these findings reveal a distinct mechanism of transcriptional induction at the hsp70 promoters, and further indicate that the apparent promoter occupancy of the general transcriptional factors does not necessarily reflect the transcriptional state of a gene. | ||
| DOI | |||
| Related Publication(s) | |||
| Supplementary material | [title not yet available] [FBrf0191585] |
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Proc. Natl. Acad. Sci. U.S.A. | ||
| Title | Proceedings of the National Academy of Sciences of the United States of America | ||
| Publication Year | 1915- | ||
| ISBN/ISSN | 0027-8424 | ||
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Data from Reference
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| Alleles (1)
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| Constructs (1)
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| Genes (26)
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| Insertions (1)
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| Natural transposons (1)
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