Reference Report

Reference
Citation	Hu, H., Li, M., Labrador, J.P., McEwen, J., Lai, E.C., Goodman, C.S., Bashaw, G.J. (2005). Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. Proc. Natl. Acad. Sci. U.S.A. 102(12): 4613--4618. (Export to RIS)
FlyBase ID	FBrf0191476
Publication Type	Research paper
PubMed ID	15755809
PubMed Abstract	The regulators of the Rho-family GTPases, GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs), play important roles in axon guidance. By means of a functional genomic study of the Rho-family GEFs and GAPs in Drosophila, we have identified a Rho-family GAP, CrossGAP (CrGAP), which is involved in Roundabout (Robo) receptor-mediated repulsive axon guidance. CrGAP physically associates with the Robo receptor. Too much or too little CrGAP activity leads to defects in Robo-mediated repulsion at the midline choice point. The CrGAP gain-of-function phenotype mimics the loss-of-function phenotypes of both Robo and Rac. Dosage-sensitive genetic interactions among CrGAP, Robo, and Rac support a model in which CrGAP transduces signals downstream of Robo receptor to regulate Rac-dependent cytoskeletal changes.
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Supplementary material	[title not yet available] [FBrf0191477]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (20)
	Cdc42^N17.Scer\UAS CdGAPr^{dsRNA.H.Scer\UAS} Mtl^Δ Rac1^J10 Rac1^N17.Scer\UAS Rac1^Scer\UAS.cLa RacGAP84C^Scer\UAS.cRa Rho1^{dsRNA.Scer\UAS} Rho1^Scer\UAS.cMa RhoGAP19D^{dsRNA.H.Scer\UAS} RhoGAP93B^{dsRNA.Scer\UAS} RhoGAP93B^EY06358 RhoGAP93B^Scer\UAS.cHa RhoGAP100F^{dsRNA.H.Scer\UAS} RhoGAPp190^{dsRNA.Scer\UAS} robo⁵ Scer\GAL4^elav.PLu Scer\GAL4^GMR.PF sli¹ tum^{dsRNA.H.Scer\UAS}
Constructs (9)
	P{GAL4-elav.L} P{GAL4-ninaE.GMR} P{UAS-Cdc42.N17} P{UAS-CrGAP.H} P{UAS-Rac1.L} P{UAS-Rac1.N17} P{UAS-Rho1.Sph} P{UAS-Rho1-dsRNA} P{UAS-RhoGAP93B-dsRNA}
Genes (14)
	Cdc42 CdGAPr Mtl Rac1 RacGAP84C Rho1 RhoGAP19D RhoGAP93B RhoGAP100F RhoGAPp190 robo Scer\GAL4 sli tum
Insertions (4)
	P{GAL4-elav.L}3A P{UAS-CrGAP.H}28 P{UAS-CrGAP.H}33 P{UAS-CrGAP.H}37