Isolation and characterization of Df(2R)BSC134
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC134 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03211 and P{XP}d05392. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f03211/ P{XP}d05392 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC134 from the segment of PBac{WH}f03211 to the left of its FRT site and the segment of P{XP}d05392 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC134 predicted from the transposable element insertions sites using release 4 coordinates are 50E1;50E6. It failed to complement Sox15KG09145 and Hsc70-5k04907. Df(2R)BSC134 heterozygotes show a severe Minute phenotype from deletion of the haploinsufficient RpS23 gene.