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Christensen, S., Cook, K. (2006.3.8). Isolation and characterization of Df(2L)BSC144. 
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Date: Wed, 08 Mar 2006  13:13:02  \-0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC144
Cc: Stacey Christensen <sjchristXXXX>, mdealXXXX, Jill Gresens
Isolation and characterization of Df(2L)BSC144
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC144 was isolated as a FLP recombinase-induced recombination
event involving PBac{RB}e01839 and P{XP}d08708. The deletion was
isolated as a chromosome lacking miniwhite markers in progeny of
P{hsFLP}1, y1 w1118; PBac{RB}e01839/P{XP}d08708 males crossed to
w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat
shocked as larvae as described in Parks et al., Nature Genetics 36:
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and
succeeding generations maintained the original genetic background of
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36:
283-287, 2004; FBrf0175002). The recombination event generated the
genetic element P+PBac{XP5.RB3}BSC144 from the segment of
PBac{RB}e01839 to the left of its FRT site and the segment of
P{XP}d08708 to the right of its FRT site. Its presence was verified
using the PCR methods and primers described in Parks et al. with the
substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the
RB3' minus primer in the Hybrid PCR protocol in the Supplementary
Methods. Exelixis, Inc. determined the insertion site of P{XP}d08708
to be at Release 3 genomic coordinate 10450004 on chromosome arm 2L.
The cytological breakpoints of Df(2L)BSC144 predicted from the
transposable element insertions sites using release 3 coordinates are
31B1;31E5. It failed to complement chico1, trk1 and bsk1.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (1)
    Alleles (3)
    Genes (3)
    Insertions (3)