Isolation and characterization of Df(2L)BSC148
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC148 was isolated as a FLP recombinase-induced recombination event involving P{XP}d05062 and PBac{WH}btvf06884. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d05062/PBac{WH}btvf06884 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC148 from the segment of P{XP}d05062 to the left of its FRT site and the segment of PBac{WH}btvf06884 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC148 predicted from the transposable element insertions sites using Release 4 coordinates are 36C8;36E3. It failed to complement BicDr5, qua1 and CadNM12.