Isolation and characterization of Df(3R)BSC140
Jill Gresens, and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC140 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07686 and PBac{WH}CG14549f01934. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B females crossed to P{hsFLP}1, y1 w1118; P{XP}d07686/PBac{WH}CG14549f01934 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC140 from the segment of P{XP}d07686 to the left of its FRT site and the segment of PBac{WH}CG14549f01934 to the right of its FRT site. The cytological breakpoints of Df(3R)BSC140 predicted from the transposable element insertions sites using release 4 coordinates are 96F1;96F10. Df(3R)BSC140 failed to complement Df(3R)Exel6204 and Df(3R)Espl3.