Isolation and characterization of Df(2L)BSC145
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC145 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG33129d11065 and PBac{WH}f02134. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG33129d11065/PBac{WH}f02134 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC145 from the segment of P{XP}CG33129d11065 to the left of its FRT site and the segment of PBac{WH}f02134 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC145 predicted from the transposable element insertions sites using Release 4 coordinates are 32C1;32C5. It failed to complement piwi06843.