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| Citation | Gresens, J., Cook, K. (2006.5.11). Isolation and characterization of Df(3L)BSC157. (Export to RIS) | ||
| FlyBase ID | FBrf0191702 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
Date: Thu, 11 May 2006 16:22:33 \-0400
To: flybase-updates@XXXXXXXXXXXXXXX From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(3L)BSC157 Cc: Stacey Christensen <sjchristXXXXXXXXXXXXXXX>, mdealXXXXXXXXXXXXXXX, Jill Gresens <jgresensXXXXXXXXXXXXXXX>, kaufmanXXXXXXXXXXXXXXX Isolation and characterization of Df(3L)BSC157 Jill Gresens and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC157 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06654 and PBac{RB}CG6765e00295. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B, Tb1 females crossed to w1118Phs-FLP; P{XP}d06654/PBac{RB}CG6765e00295 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC157 from the segment of P{XP}d06654 to the left of its FRT site and the segment of PBac{RB}CG6765e00295 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d06654 to be at Release 3 genomic coordinate 8380767 on chromosome arm 3L. The cytological breakpoints of Df(3L)BSC157 predicted from the transposable element insertions sites using Release 4 coordinates are 66C12;66D5. It failed to complement Gug03928. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (1)
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| Genes (1)
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| Insertions (3)
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