Reference Report

Reference
Citation	Loncle, N., Boube, M., Joulia, L., Boschiero, C., Werner, M., Cribbs, D.L., Bourbon, H.M. (2007). Distinct roles for Mediator Cdk8 module subunits in Drosophila development. EMBO J. 26(4): 1045--1054. (Export to RIS)
FlyBase ID	FBrf0191850
Publication Type	Research paper
PubMed ID	17290221
PubMed Abstract	Mediator (MED) is a conserved multisubunit complex bridging transcriptional activators and repressors to the general RNA polymerase II initiation machinery. In yeast, MED is organized in three core modules and a separable 'Cdk8 module' consisting of the cyclin-dependent kinase Cdk8, its partner CycC, Med12 and Med13. This regulatory module, specifically required for cellular adaptation to environmental cues, is thought to act through the Cdk8 kinase activity. Here we have investigated the functions of the four Cdk8 module subunits in the metazoan model Drosophila. Physical interactions detected among the four fly subunits provide support for a structurally conserved Cdk8 module. We analyzed the in vivo functions of this module using null mutants for Cdk8, CycC, Med12 and Med13. Each gene is required for the viability of the organism but not of the cell. Cdk8-CycC and Med12-Med13 act as pairs, which share some functions but also have distinct roles in developmental gene regulation. These data reveal functional attributes of the Cdk8 module, apart from its regulated kinase activity, that may contribute to the diversification of genetic programs.
DOI	10.1038/sj.emboj.7601566
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (6)
	Df(3L)AC1 Df(3L)Cdk8^K145 Df(3L)Cdk8^K185 Df(3R)CycC^Y5 Df(3R)CycC^Y8 Df(3R)Exel6172
Alleles (16)
	Cdk8^K185 Cdk8^Ubi-p63E.PL CG3731^Ubi-p63E.PL CG33332^Ubi-p63E.PL CycC^GE22979 CycC^Scer\UAS.cLa CycC^Y5 I-2^GE26681 I-2^K9 kto^T241 Scer\FLP1^ey.PB Scer\FLP1^Scer\UAS.cUa Scer\GAL4^ap-md544 Scer\GAL4^arm.PS Scer\GAL4^Dll-em212 skd^T606
Constructs (9)
	P{?EP} P{EP} P{ey-FLP.B} P{GAL4-arm.S} P{UAS-CycC.L} P{UAS-FLP1.U} P{Ubi-p63E-Cdk8.L} P{Ubi-p63E-CG3731.L} P{Ubi-p63E-CG33332.L}
Genes (17)
	bab2 Cdk8 CG3731 CG33331 CG33332 CycC dac Dll dpp I-2 kto RasGAP1 Scer\FLP1 Scer\GAL4 sens skd wg
Insertions (9)
	P{?EP}I-2^GE26681 P{EP}CycC^GE22979 P{EP}I-2^GE26681 P{GAL4-arm.S}11 P{GawB}ap^md544 P{GawB}Dll^em212 P{UAS-CycC.L}wdE P{Ubi-p63E-Cdk8.L}1.14 P{Ubi-p63E-CG3731.L}F41.2
Natural transposons (1)
	P-element