Reference Report

Reference
Citation	Gallagher, C.M., Knoblich, J.A. (2006). The conserved c2 domain protein lethal (2) giant discs regulates protein trafficking in Drosophila. Dev. Cell 11(5): 641--653. (Export to RIS)
FlyBase ID	FBrf0192039
Publication Type	Research paper
PubMed ID	17084357
PubMed Abstract	Drosophila sensory organ precursor (SOP) cells undergo several rounds of asymmetric cell division to generate the four different cell types that make up external sensory organs. Establishment of different fates among daughter cells of the SOP relies on differential regulation of the Notch pathway. Here, we identify the protein Lethal (2) giant discs (Lgd) as a critical regulator of Notch signaling in the SOP lineage. We show that lgd encodes a conserved C2 domain protein that binds to phospholipids present on early endosomes. When Lgd function is compromised, Notch and other transmembrane proteins accumulate in enlarged early endosomal compartments. These enlarged endosomes are positive for Rab5 and Hrs, a protein involved in trafficking into the degradative pathway. Our experiments suggest that Lgd is a critical regulator of endocytosis that is not present in yeast and acts in the degradative pathway after Hrs.
DOI	10.1016/j.devcel.2006.09.014
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (1)
	Df(2L)FCK-20
Alleles (30)
	Avic\GFP^{Scer\UAS.T:Hsap\MYC,T:Mmus\2xFYVE} crol^EP1183 Hrs^D28 l(2)gd1¹ l(2)gd1⁴ l(2)gd1⁰⁸ l(2)gd1²⁴ l(2)gd1^d7 l(2)gd1^d10 l(2)gd1^Scer\UAS.cGa l(2)gd1^{Scer\UAS.T:Hsap\MYC} l(2)gd1^{ΔC2.C.Scer\UAS.T:Zzzz\FLAG} l(2)gd1^{ΔC2.C.Scer\UAS} l(2)gd1^{ΔC2.N.Scer\UAS.T:Avic\GFP} l(2)gd1^{ΔC2.N.Scer\UAS.T:Zzzz\FLAG} l(2)gd1^{ΔC2.Scer\UAS} l(2)gd1^{ΔN.C.Scer\UAS.T:Avic\GFP} l(2)gd1^{ΔN.C.Scer\UAS.T:Zzzz\FLAG} l(2)gd1^{ΔN.N.Scer\UAS.T:Avic\GFP} l(2)gd1^{ΔN.N.Scer\UAS.T:Zzzz\FLAG} l(2)gd1^ΔN.Scer\UAS N^l1N-ts1 ppo^EP2478 Rab5^{Scer\UAS.T:Avic\GFP-EGFP} Rab7^{Scer\UAS.T:Avic\GFP} Rab11^{Scer\UAS.T:Avic\GFP} Scer\GAL4^MS248 Scer\GAL4^neur-P72 Scer\GAL4^sca-109-68 sec15^{C.Scer\UAS.T:Avic\GFP}
Constructs (17)
	P{UAS-GFP-myc-2xFYVE} P{UAS-GFP-Rab5} P{UAS-l(2)gd1.2xmyc} P{UAS-l(2)gd1.G} P{UAS-l(2)gd1.ΔC2.C.3xFlag} P{UAS-l(2)gd1.ΔC2.C.GFP} P{UAS-l(2)gd1.ΔC2.N.3xFlag} P{UAS-l(2)gd1.ΔC2.N.GFP} P{UAS-l(2)gd1.ΔC2} P{UAS-l(2)gd1.ΔN.C.3xFlag} P{UAS-l(2)gd1.ΔN.C.GFP} P{UAS-l(2)gd1.ΔN.N.3xFlag} P{UAS-l(2)gd1.ΔN.N.GFP} P{UAS-l(2)gd1.ΔN} P{UAS-Rab7.GFP} P{UAS-Rab11.GFP} P{UAS-sec15.GFP}
Genes (24)
	ase Avic\GFP crol ct Dl Egfr elav Hrs l(2)gd1 N numb pon ppo pros Rab5 Rab7 Rab11 Scer\GAL4 sec15 Su(H) Syx1A T:Avic\GFP T:Hsap\MYC T:Zzzz\FLAG
Insertions (5)
	P{EP}crol^EP1183 P{EP}ppo^EP2478 P{GAL4}neur^P72 P{GawB}MS248 P{GawB}sca^109-68
Natural transposons (1)
	P-element