Reference Report

Reference
Citation	McCaffrey, R., St Johnston, D., Gonzalez-Reyes, A. (2006). Drosophila mus301/spindle-C encodes a helicase with an essential role in double-strand DNA break repair and meiotic progression. Genetics 174(3): 1273--1285. (Export to RIS)
FlyBase ID	FBrf0192058
Publication Type	Research paper
External Crossreferences
PubMed ID	16888338
PubMed Abstract	mus301 was identified independently in two genetic screens, one for mutants hypersensitive to chemical mutagens and another for maternal mutants with eggshell defects. mus301 is required for the proper specification of the oocyte and for progression through meiosis in the Drosophila ovary. We have cloned mus301 and show that it is a member of the Mus308 subfamily of ATP-dependent helicases and the closest homolog of human and mouse HEL308. Functional analyses demonstrate that Mus301 is involved in chromosome segregation in meiosis and in the repair of double-strand-DNA breaks in both meiotic and mitotic cells. Most of the oogenesis defects of mus301 mutants are suppressed by mutants in the checkpoint kinase Mei41 and in MeiW68, the Spo11 homolog that is thought to generate the dsDNA breaks that initiate recombination, indicating that these phenotypes are caused by activation of the DNA damage checkpoint in response to unrepaired Mei-W68-induced dsDNA breaks. However, neither mei-W68 nor mei-41 rescue the defects in oocyte specification of mus301 mutants, suggesting that this helicase has another function in oocyte selection that is independent from its role in meiotic recombination.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Editors
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(2L)pr65 Df(2R)LL5 Df(3L)66C-G28 Df(3L)ZP3
Alleles (18)
	grp^fsA4 lok^p6 mei-41^D3 mei-W68¹ mus301⁰⁹⁴ mus301⁴²² mus301⁶⁶⁰ mus301^+t6.2 mus301^D1 mus301^D2 mus301^D4 mus301^ZIII-2255 mus301^ZIII-3198 mus301^ZIII-4875 spn-D¹ spn-D² spn-E¹ spn-E²
Constructs (1)
	P{mus301^+t6.2}
Genes (14)
	Act5C Act42A c(3)G grk grp His2Av lok mei-41 mei-W68 mus301 mus308 orb spn-D spn-E
Insertions (1)
	P{lacW}l(3)S090314^S090314
Natural transposons (1)
	P-element