Reference Report

Reference
Citation	Verdier, V., Chen, G.C., Settleman, J. (2006). Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophila development. BMC Dev. Biol. 6(1): 38. (Export to RIS)
FlyBase ID	FBrf0192322
Publication Type	Research paper
PubMed ID	16882341
PubMed Abstract	The Rho-kinases (ROCKs) are major effector targets of the activated Rho GTPase that have been implicated in many of the Rho-mediated effects on cell shape and movement via their ability to affect acto-myosin contractility. The role of ROCKs in cell shape change and motility suggests a potentially important role for Rho-ROCK signaling in tissue morphogenesis during development. Indeed, in Drosophila, a single ROCK ortholog, DRok, has been identified and has been found to be required for establishing planar cell polarity.We have examined a potential role for DRok in additional aspects of tissue morphogenesis using an activated form of the protein in transgenic flies. Our findings demonstrate that DRok activity can influence multiple morphogenetic processes, including eye and wing development. Furthermore, genetic studies reveal that Drok interacts with multiple downstream effectors of the Rho GTPase signaling pathway, including non-muscle myosin heavy chain, adducin, and Diaphanous in those developmental processes. Finally, in overexpression studies, we determined that Drok and Drosophila Lim-kinase interact in the developing nervous system.These findings indicate widespread diverse roles for DRok in tissue morphogenesis during Drosophila development, in which multiple DRok substrates appear to be required.
DOI	10.1186/1471-213X-6-38
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Language of Publication	English
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Publication Type	Journal
Abbreviation	BMC Dev. Biol.
Title	BMC Developmental Biology
Publication Year	2002
ISBN/ISSN	1471-213X
Data from Reference
Alleles (22)
	BacA\p35^GMR.PH dia^k07135 hts⁰¹¹⁰³ LIMK1^GMR.PV LIMK1^Scer\UAS.cCa Rho1^72O rok^cat.GMR rok^cat.Scer\UAS Scer\GAL4^69B Scer\GAL4^Act5C.PU Scer\GAL4^Cy6 Scer\GAL4^da.G32 Scer\GAL4^elav.PU Scer\GAL4^en-e16E Scer\GAL4^ey.PU Scer\GAL4^GMR.PU Scer\GAL4^insc-Mz1407 Scer\GAL4^LE Scer\GAL4^prd.RG1 Scer\GAL4^tub.PU sqh² zip¹
Constructs (12)
	P{Act5C-GAL4.U} P{elav-GAL4.U} P{ey-GAL4.U} P{GAL4-da.G32} P{GAL4-prd.F} P{GMR-GAL4.U} P{GMR-LIMK1.V} P{GMRP35} P{GMR-rok.cat} P{tub-GAL4.U} P{UAS-LIMK1.C} P{UAS-rok.cat}
Genes (13)
	BacA\p35 cv cv-2 dia dpp gbb hts LIMK1 Rho1 rok Scer\GAL4 sqh zip
Insertions (5)
	P{en2.4-GAL4}e16E P{GAL4}Cy6 P{GAL4}LE P{GawB}69B P{GawB}insc^Mz1407
Natural transposons (1)
	P-element