Reference Report

Reference
Citation	Zhang, Y., Guo, H., Kwan, H., Wang, J.W., Kosek, J., Lu, B. (2007). PAR-1 kinase phosphorylates Dlg and regulates its postsynaptic targeting at the Drosophila neuromuscular junction. Neuron 53(2): 201--215. (Export to RIS)
FlyBase ID	FBrf0192396
Publication Type	Research paper
PubMed ID	17224403
PubMed Abstract	Targeting of synaptic molecules to their proper location is essential for synaptic differentiation and plasticity. PSD-95/Dlg proteins have been established as key components of the postsynapse. However, the molecular mechanisms regulating the synaptic targeting, assembly, and disassembly of PSD-95/Dlg are not well understood. Here we show that PAR-1 kinase, a conserved cell polarity regulator, is critically involved in controlling the postsynaptic localization of Dlg. PAR-1 is prominently localized at the Drosophila neuromuscular junction (NMJ). Loss of PAR-1 function leads to increased synapse formation and synaptic transmission, whereas overexpression of PAR-1 has the opposite effects. PAR-1 directly phosphorylates Dlg at a conserved site and negatively regulates its mobility and targeting to the postsynapse. The ability of a nonphosphorylatable Dlg to largely rescue PAR-1-induced synaptic defects supports the idea that Dlg is a major synaptic substrate of PAR-1. Control of Dlg synaptic targeting by PAR-1-mediated phosphorylation thus constitutes a critical event in synaptogenesis.
DOI	10.1016/j.neuron.2006.12.016
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Alleles (13)
	dlg1⁷ dlg1^{SA.Scer\UAS.T:Avic\GFP} dlg1^{SD.Scer\UAS.T:Avic\GFP} dlg1^{WT.Scer\UAS.T:Avic\GFP} par-1^{dsRNA.Scer\UAS.cZa} par-1^k06323 par-1^KN.Scer\UAS par-1^{N1S.Scer\UAS.P\T.T:Avic\GFP-m6} par-1^Scer\UAS.cSa par-1^W3 par-1^Δ-16 Scer\GAL4^elav.PU Scer\GAL4^Mhc.PW
Constructs (9)
	P{elav-GAL4.U} P{GAL4-Mhc.W} P{UAS-dlg1.SA.GFP} P{UAS-dlg1.SD.GFP} P{UAS-dlg1.WT.GFP} P{UAS-par-1.KN} P{UAS-par-1.RNAi.Z} P{UAS-par-1.S} P{UASp-par-1.N1S.GFP}
Genes (6)
	Csp dlg1 GluRIIA par-1 Scer\GAL4 T:Avic\GFP
Natural transposons (1)
	P-element