Reference Report

Reference
Citation	Awasaki, T., Tatsumi, R., Takahashi, K., Arai, K., Nakanishi, Y., Ueda, R., Ito, K. (2006). Essential role of the apoptotic cell engulfment genes draper and ced-6 in programmed axon pruning during Drosophila metamorphosis. Neuron 50(6): 855--867. (Export to RIS)
FlyBase ID	FBrf0192438
Publication Type	Research paper
PubMed ID	16772168
PubMed Abstract	Axon pruning is a common phenomenon in neural circuit development. Previous studies demonstrate that the engulfing action of glial cells is essential in this process. The underlying molecular mechanisms, however, remain unknown. We show that draper (drpr) and ced-6, which are essential for the clearance of apoptotic cells in C. elegans, function in the glial engulfment of larval axons during Drosophila metamorphosis. The drpr mutation and glia-specific knockdown of drpr and ced-6 by RNA interference suppress glial engulfment, resulting in the inhibition of axon pruning. drpr and ced-6 interact genetically in the glial action. Disruption of the microtubule cytoskeleton in the axons to be pruned occurs via ecdysone signaling, independent of glial engulfment. These findings suggest that glial cells engulf degenerating axons through drpr and ced-6. We propose that apoptotic cells and degenerating axons of living neurons are removed by a similar molecular mechanism.
DOI	10.1016/j.neuron.2006.04.027
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Alleles (13)
	Act5C^{Scer\UAS.T:Avic\GFP} Avic\GFP^{S65T.Scer\UAS} BacA\p35^Scer\UAS.cHa ced-6^{dsRNA.Scer\UAS.cAa} drpr^{dsRNA.Scer\UAS.cAa} drpr^Δ5 Ecol\lacZ^{Scer\UAS.T:SV40\nls2} EcR^{B1-ΔC655.F645A.Scer\UAS} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^repo Scer\GAL4^Tab2-201Y th^Scer\UAS.cHa αTub84B^{Scer\UAS.T:Hsap\MYC}
Constructs (10)
	P{UAS-Act5C.T:GFP} P{UAS-ced-6.IR.A} P{UAS-DIAP1.H} P{UAS-drpr.IR.A} P{UAS-EcR.B1-ΔC655.F645A} P{UAS-GFP.S65T} P{UAS-lacZ.NZ} P{UAS-mCD8::GFP.L} P{UAS-p35.H} P{UAS-αTub84B.Myc}
Genes (15)
	Act5C Avic\GFP BacA\p35 ced-6 drpr Ecol\lacZ EcR elav Fas2 Mmus\Cd8a repo Scer\GAL4 th trio αTub84B
Insertions (2)
	P{GAL4}repo P{GawB}Tab2^201Y
Natural transposons (1)
	P-element